Alberio Tiziana, Brughera Martina, Lualdi Marta
Department of Science and High Technology and Center of Neuroscience, University of Insubria, I-21052 Busto Arsizio, VA, Italy.
Biomedicines. 2022 Sep 15;10(9):2297. doi: 10.3390/biomedicines10092297.
The growing number of patients affected by neurodegenerative disorders represents a huge problem for healthcare systems, human society, and economics. In this context, omics strategies are crucial for the identification of molecular factors involved in disease pathobiology, and for the discovery of biomarkers that allow early diagnosis, patients' stratification, and treatment response prediction. The integration of different omics data is a required step towards the goal of personalized medicine. The Italian proteomics community is actively developing and applying proteomics approaches to the study of neurodegenerative disorders; moreover, it is leading the mitochondria-focused initiative of the Human Proteome Project, which is particularly important given the central role of mitochondrial impairment in neurodegeneration. Here, we describe how Italian research groups in proteomics have contributed to the knowledge of many neurodegenerative diseases, through the elucidation of the pathobiology of these disorders, and through the discovery of disease biomarkers. In particular, we focus on the central role of post-translational modifications analysis, the implementation of network-based approaches in functional proteomics, the integration of different omics in a systems biology view, and the development of novel platforms for biomarker discovery for the high-throughput quantification of thousands of proteins at a time.
受神经退行性疾病影响的患者数量不断增加，这对医疗保健系统、人类社会和经济来说都是一个巨大的问题。在这种背景下，组学策略对于识别参与疾病病理生物学的分子因素以及发现能够实现早期诊断、患者分层和治疗反应预测的生物标志物至关重要。整合不同的组学数据是迈向个性化医疗目标的必要步骤。意大利蛋白质组学界正在积极开发并应用蛋白质组学方法来研究神经退行性疾病；此外，它还引领着人类蛋白质组计划中以线粒体为重点的倡议，鉴于线粒体损伤在神经退行性变中的核心作用，这一点尤为重要。在此，我们描述了意大利蛋白质组学研究团队如何通过阐明这些疾病的病理生物学以及发现疾病生物标志物，为多种神经退行性疾病的认识做出贡献。特别是，我们重点关注翻译后修饰分析的核心作用、功能蛋白质组学中基于网络方法的实施、系统生物学视角下不同组学的整合，以及开发用于生物标志物发现的新型平台，以便能够一次对数千种蛋白质进行高通量定量分析。
