Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Hangtian West Road, Guiyang 550025, China.
Guizhou Institute of Walnut, 214 Fuyuan South Road, Guiyang 550005, China.
Genes (Basel). 2022 Sep 10;13(9):1628. doi: 10.3390/genes13091628.
(Iwade) Yokoyama is a species of wild fungi that grows in southwest China, considered an edible and medicinal fungus with potential commercial prospects. However, the detailed mechanisms related to the development of mycelium and the formation of the fruiting body are unclear. To obtain a comprehensive overview of genetic features, whole-genome and comparative genomics analyses of were performed. High-quality DNA was extracted from the mycelium, which was isolated from a fresh fruiting body of . The DNA sample was subjected to sequencing using Illumina and Oxford Nanopore sequencing platforms. A genome assembly totaling 40.15 Mb in 50 contigs with an N50 length of 2.06 Mb was generated, and 8705 putative predicted genes were found. Subsequently, phylogenetic analysis revealed a close evolutionary relationship between and . Moreover, a total of 403 carbohydrate-active enzymes (CAZymes) were identified in , which included 147 glycoside hydrolases (GHs), 85 glycosyl transferases (GTs), 8 polysaccharide lyases (PLs), 76 carbohydrate esterases (CEs), 57 auxiliary activities (AAs) and 30 carbohydrate-binding modules (CBMs). Compared with the other 13 fungi (, , , etc.), the number and distribution of CAZymes in were similar to other mycorrhizal fungi. Furthermore, the optimization of culture medium for showed the efficient utilization of disaccharides such as sucrose and maltose. The genome of provides new insights into its niche, food applications and potential artificial domestication.
（岩垂）山野生菌是一种生长在中国西南部的野生真菌，被认为是一种可食用和药用真菌，具有潜在的商业前景。然而，关于其菌丝体发育和子实体形成的详细机制尚不清楚。为了全面了解其遗传特征，对其进行了全基因组和比较基因组分析。从新鲜子实体中分离出的菌丝体中提取了高质量的 DNA。使用 Illumina 和 Oxford Nanopore 测序平台对 DNA 样本进行测序。共组装出 50 条大小为 40.15Mb 的 contigs，N50 长度为 2.06Mb，共预测到 8705 个基因。随后，系统发育分析表明与亲缘关系密切。此外，共鉴定出 403 种碳水化合物活性酶（CAZymes），包括 147 种糖苷水解酶（GHs）、85 种糖基转移酶（GTs）、8 种多糖裂解酶（PLs）、76 种糖酯酶（CEs）、57 种辅助活性酶（AAs）和 30 种碳水化合物结合模块（CBMs）。与其他 13 种真菌（，，，等）相比，在中的数量和分布与其他菌根真菌相似。此外，优化了的培养基，显示出对蔗糖和麦芽糖等二糖的有效利用。的基因组为其生态位、食品应用和潜在的人工驯化提供了新的见解。
