Abdallah-Ruiz Angelica, Wood Lurdes Siberio, Kim Taejo, Schilling Wes, White Shecoya B, Chen Bang-Yuan, Durango-Villadiego Alba, Silva Juan L
Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS 39762, USA.
Department of Kinesiology, Health, Food and Nutrition Sciences, University of Wisconsin-Stout, Menomonie, WI 54751, USA.
Foods. 2022 Sep 9;11(18):2778. doi: 10.3390/foods11182778.
The catfish industry is important to the United States economy. The present study determined the levels of microbial indicators and the prevalence of Listeria spp. and Listeria monocytogenes at catfish farms and catfish processing plants. Live fish, water, and sediment samples were analyzed in farms. Fish skin, fillets, chiller water, and environmental surfaces were assessed at the processing plants both during operation and after sanitation. Live fish had 2% prevalence of Listeria monocytogenes, while sediment and water were negative for Listeria. Live fish skin counts averaged 4.2, 1.9, and 1.3 log CFU/cm2 aerobic (APC), total coliform (TCC) and generic Escherichia coli counts, respectively. Water and sediment samples averaged 4.8 and 5.8 log CFU/g APC, 1.9 and 2.3 log CFU/g TCC, and 1.0 and 1.6 log CFU/g generic E. coli counts, respectively. During operation, Listeria prevalence was higher in fillets before (57%) and after (97%) chilling than on fish skin (10%). Process chiller water had higher (p ≤ 0.05) APC, TCC, and Listeria prevalence than clean chiller water. After sanitation, most sampling points in which Listeria spp. were present had high levels of APC (>2.4 log CFU/100 cm2). APC combined with Listeria spp. could be a good approach to understand microbial contamination in catfish plants.
鲶鱼产业对美国经济很重要。本研究确定了鲶鱼养殖场和鲶鱼加工厂中微生物指标的水平以及李斯特菌属和单核细胞增生李斯特菌的流行情况。在养殖场对活鱼、水和沉积物样本进行了分析。在加工厂运营期间和卫生处理后，对鱼皮、鱼片、冷却用水和环境表面进行了评估。活鱼中单核细胞增生李斯特菌的流行率为2%，而沉积物和水中未检测到李斯特菌。活鱼皮肤的需氧菌（APC）、总大肠菌群（TCC）和大肠埃希氏菌属的平均计数分别为4.2、1.9和1.3 log CFU/cm²。水和沉积物样本的APC平均计数分别为4.8和5.8 log CFU/g，TCC分别为1.9和2.3 log CFU/g，大肠埃希氏菌属分别为1.0和1.6 log CFU/g。在运营期间，冷却前（57%）和冷却后（97%）的鱼片李斯特菌流行率高于鱼皮（10%）。加工冷却用水的APC、TCC和李斯特菌流行率高于清洁冷却用水（p≤0.05）。卫生处理后，存在李斯特菌属的大多数采样点的APC水平较高（>2.4 log CFU/100 cm²）。APC与李斯特菌属相结合可能是了解鲶鱼加工厂微生物污染的一个好方法。
