对食品加工环境中推定分离株的分子特征进行调查。
The investigation of molecular characterization of presumptive isolates from a food-processing environment.
作者信息
Ahmed M S
机构信息
Duhok Research Centre, College of Veterinary Medicine, University of Duhok, Duhok, Iraq.
出版信息
Iran J Vet Res. 2019 Winter;20(1):46-50.
BACKGROUND
is a Gram-positive, non-spore forming, facultative anaerobic intracellular bacterium. The most important pathogens in mammals include and . The former generally causes disease and death in both humans and animals while the latter performs sporadically and primarily causes illness in ruminants.
AIMS
The aim of this project was to use conventional and molecular techniques to determine whether the provided samples were , and whether they were genetically similar or different.
METHODS
The provided presumptive cultures isolated from industrial processed food are conventionally assumed to be . All samples were cultured on brain heart infusion agar and broth first and then on blood agar. Later, gene amplification was applied.
RESULTS
The provided culture phenotypically resembled as it caused haemolysis on blood agar plates; however, the absence of the gene revealed that they were genotypically different. confirmed three species of species including , and . The results from sequencing confirmed the results obtained from gene amplification.
CONCLUSION
Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC PCR) confirmed that all bacterial cultures were isolated from different sources depending on their ERIC PCR profile variation.
背景
是一种革兰氏阳性、不形成芽孢、兼性厌氧的细胞内细菌。哺乳动物中最重要的病原体包括和。前者通常在人类和动物中都导致疾病和死亡，而后者偶尔致病，主要导致反刍动物患病。
目的
本项目的目的是使用传统和分子技术来确定所提供的样本是否为，以及它们在基因上是相似还是不同。
方法
从工业加工食品中分离出的推定培养物通常被认为是。所有样本首先在脑心浸液琼脂和肉汤中培养，然后在血琼脂上培养。随后，进行基因扩增。
结果
所提供的培养物在表型上类似于，因为它在血琼脂平板上引起溶血；然而，基因的缺失表明它们在基因型上是不同的。确认了三种物种，包括、和。测序结果证实了从基因扩增获得的结果。
结论
肠杆菌重复基因间共识聚合酶链反应（ERIC PCR）证实，所有细菌培养物根据其ERIC PCR图谱变化从不同来源分离得到。
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