Hentzen D, Renucci A, le Guellec D, Benchaibi M, Jurdic P, Gandrillon O, Samarut J
Mol Cell Biol. 1987 Jul;7(7):2416-24. doi: 10.1128/mcb.7.7.2416-2424.1987.
We analyzed the expression of the c-erbA proto-oncogene in different tissues of chicken embryos. c-erbA transcripts were found at low levels in the lung, kidney, liver, and heart and in high amounts in embryonic blood cells. Nuclease mapping assays proved that these transcripts were true c-erbA transcripts. In situ hybridization on fractionated embryonic blood cells showed that c-erbA transcripts were predominantly found in erythroblasts, particularly during the final step of differentiation. Life span analysis of c-erbA mRNAs revealed their relative instability, demonstrating that the high level of c-erbA transcripts in embryonic erythroblasts was not the result of passive accumulation. These results suggest that the c-erbA genes play some role in erythrocyte differentiation.
我们分析了鸡胚不同组织中c-erbA原癌基因的表达情况。在肺、肾、肝和心脏中发现c-erbA转录本水平较低，而在胚胎血细胞中含量较高。核酸酶图谱分析证明这些转录本是真正的c-erbA转录本。对分离的胚胎血细胞进行原位杂交显示，c-erbA转录本主要存在于成红细胞中，尤其是在分化的最后阶段。对c-erbA mRNA的寿命分析揭示了它们相对不稳定，这表明胚胎成红细胞中c-erbA转录本的高水平并非被动积累的结果。这些结果表明c-erbA基因在红细胞分化中发挥了一定作用。
