Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Department of Biotechnology and Animal Science, National Ilan University, Ilan 260, Taiwan.
Int J Mol Sci. 2022 Sep 8;23(18):10392. doi: 10.3390/ijms231810392.
The edible fungus was shown to have a high molecular weight (1.87 × 10 kDa) bioactive polysaccharide, denoted as TFP-F1. Monosaccharide composition and NMR analysis of the polysaccharide and its derivatives indicated it contained fucose (Fuc), xylose (Xyl), mannose (Man), and glucuronic acid (GlcA) in a ratio of 0.9:1.0:3.2:1.2. Using IR, NMR, and GC-MS spectroscopic data, the structure of TFP-F1 was elucidated as {→3)-[-D-GlcA-(1→2)]--D-Man-(1→3)--D-Man-(1→3)-[-L-Fuc-(1→2)--D-Xyl-(1→2)]--D-Man-(1→}, with partial acetylation of C6-OH in mannoses. Furthermore, at a concentration of 1 μg/mL, TFP-F1 was found to stimulate the secretion of TNF-α and IL-6 in J774A.1 macrophage cells in vitro via interaction with toll-like receptor 4 (TLR4). The removal of -acetyl groups led to the loss of immunomodulatory activities, demonstrating that -acetyl groups play an essential role in enhancing the production of pro-inflammatory cytokines.
该食用真菌表现出高分子量（1.87×10 kDa）的生物活性多糖，命名为 TFP-F1。多糖及其衍生物的单糖组成和 NMR 分析表明，它含有岩藻糖（Fuc）、木糖（Xyl）、甘露糖（Man）和葡萄糖醛酸（GlcA），比例为 0.9:1.0:3.2:1.2。利用 IR、NMR 和 GC-MS 光谱数据，确定了 TFP-F1 的结构为{→3）-[-D-GlcA-(1→2)]--D-Man-(1→3)--D-Man-(1→3)-[-L-Fuc-(1→2)--D-Xyl-(1→2)]--D-Man-(1→}，甘露糖 C6-OH 部分乙酰化。此外，在 1μg/mL 的浓度下，TFP-F1 被发现能够通过与 toll 样受体 4（TLR4）相互作用，刺激 J774A.1 巨噬细胞细胞体外分泌 TNF-α和 IL-6。-乙酰基的去除导致免疫调节活性丧失，表明 -乙酰基在增强促炎细胞因子的产生中起重要作用。
