Zhou Shipeng, Tan Qiuhua, Wen Bingjian, Bai Yan, Che Qishi, Cao Hua, Guo Jiao, Su Zhengquan
Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Metabolites. 2022 Sep 15;12(9):867. doi: 10.3390/metabo12090867.
Alcoholic liver disease (ALD) is a primary cause of mortality and morbidity worldwide. Oxidative stress and inflammation are important pathogenic factors contributing to ALD. We investigated the protective mechanism of galacto-oligosaccharide (GOS) against ALD through their antioxidant and anti-inflammatory activities by performing in vivo and in vitro experiments. Western blot and RT‒PCR results indicated that the expression of cytochrome P450 protein 2E1 (CYP2E1) in liver tissues and L02 cells was reduced in the GOS-treated mice compared with the model group. In addition, GOS prominently reduced the expression of Kelch-like ECH-associated protein 1 (Keap1), increased the expression of the nuclear factor erythroid-2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1) proteins, and enhanced the antioxidant capacity. In addition, GOS decreased inflammation by reducing inflammatory factor levels and inhibiting the mitogen-activated protein kinase (MAPK)/nuclear factor kappa B (NF-κB) pathway. Based on these results, GOS may be a prospective functional food for the prevention and treatment of ALD.
酒精性肝病（ALD）是全球范围内导致死亡和发病的主要原因。氧化应激和炎症是导致ALD的重要致病因素。我们通过体内和体外实验，研究了低聚半乳糖（GOS）通过其抗氧化和抗炎活性对ALD的保护机制。蛋白质免疫印迹法和逆转录-聚合酶链反应结果表明，与模型组相比，GOS处理的小鼠肝组织和L02细胞中细胞色素P450蛋白2E1（CYP2E1）的表达降低。此外，GOS显著降低了类 Kelch 样环氧氯丙烷相关蛋白1（Keap1）的表达，增加了核因子红细胞2相关因子2（Nrf2）和血红素加氧酶-1（HO-1）蛋白的表达，并增强了抗氧化能力。此外，GOS通过降低炎症因子水平和抑制丝裂原活化蛋白激酶（MAPK）/核因子κB（NF-κB）途径来减轻炎症。基于这些结果，GOS可能是一种用于预防和治疗ALD的潜在功能性食品。
