Carrasco-Espinosa Karen, Avitia Morena, Barrón-Sandoval Alberto, Abbruzzini Thalita F, Salazar Cabrera Ulises Isaac, Arroyo-Lambaer Denise, Uscanga Adriana, Campo Julio, Benítez Mariana, Wegier Ana, Rosell Julieta A, Reverchon Frédérique, Hernández Gerardo, Boege Karina, Escalante Ana E
Laboratorio Nacional de Ciencias de la Sostenibilidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Microorganisms. 2022 Aug 31;10(9):1763. doi: 10.3390/microorganisms10091763.
Despite the central role of microorganisms in soil fertility, little understanding exists regarding the impact of management practices and soil microbial diversity on soil processes. Strong correlations among soil microbial composition, management practices, and microbially mediated processes have been previously shown. However, limited integration of the different parameters has hindered our understanding of agroecosystem functioning. Multivariate analyses of these systems allow simultaneous evaluation of the parameters and can lead to hypotheses on the microbial groups involved in specific nutrient transformations. In the present study, using a multivariate approach, we investigated the effect of microbial composition (16SrDNA sequencing) and soil properties in carbon mineralization (C) (BIOLOG™, Hayward, CA, USA) across different management categories on coffee agroecosystems in Mexico. Results showed that (i) changes in soil physicochemical variables were related to management, not to region, (ii) microbial composition was associated with changes in management intensity, (iii) specific bacterial groups were associated with different management categories, and (iv) there was a broader utilization range of carbon sources in non-managed plots. The identification of specific bacterial groups, management practices, and soil parameters, and their correlation with the utilization range of carbon sources, presents the possibility to experimentally test hypotheses on the interplay of all these components and further our understanding of agroecosystem functioning and sustainable management.
尽管微生物在土壤肥力中起着核心作用，但对于管理措施和土壤微生物多样性对土壤过程的影响，人们了解甚少。此前已表明土壤微生物组成、管理措施和微生物介导过程之间存在强相关性。然而，不同参数的有限整合阻碍了我们对农业生态系统功能的理解。对这些系统进行多变量分析可以同时评估参数，并能得出关于参与特定养分转化的微生物群体的假设。在本研究中，我们采用多变量方法，研究了微生物组成（16SrDNA测序）和土壤性质对墨西哥咖啡农业生态系统不同管理类别下碳矿化（C）（美国加利福尼亚州海沃德市的BIOLOG™）的影响。结果表明：（i）土壤理化变量的变化与管理措施有关，而非与区域有关；（ii）微生物组成与管理强度的变化有关；（iii）特定细菌群体与不同管理类别有关；（iv）未管理地块中碳源的利用范围更广。特定细菌群体、管理措施和土壤参数的确定，以及它们与碳源利用范围的相关性，为通过实验检验所有这些组分之间相互作用的假设以及进一步理解农业生态系统功能和可持续管理提供了可能性。
