A Plasmid Carrying in Belonging to a Novel Resistance Plasmid Family.

and are the most detected plasmid-encoded carbapenemase genes in . Previous studies have reported plasmid sequences carrying variants, except . In this study, we aimed to characterize a plasmid carrying in a strain isolated from a Mexican hospital. The whole genome of strain PE52 was sequenced using Illumina Miseq 2 × 150 bp, with 5 million paired-end reads. We characterized a 27 kb plasmid (pPE52IMP) that carried . The phylogenetic analysis of RepA in pPE52IMP and 33 plasmids carrying resistance genes reported in the GenBank revealed that pPE52IMP and four plasmids (pMATVIM-7, unnamed (FDAARGOS_570), pD5170990, and pMRVIM0713) were in the same clade. These closely related plasmids belonged to the MOB subfamily and had similar backbones. Another plasmid (p4130-KPC) had a similar backbone to pPE52IMP; however, its RepA was truncated. In these plasmids, the resistance genes , variants, , variants, and were inserted between and resolvase genes. This study describes a new family of plasmids carrying resistance genes, with a similar backbone, the same RepA, and belonging to the MOB subfamily in . In addition, our characterized plasmid harboring (pPE52IMP) belongs to this family.