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子宫体腺癌中雌激素受体酶免疫测定法与放射配体结合测定法(等电聚焦)的比较。

Comparison of estrogen receptor enzyme immunoassay and a radioligand binding assay (isoelectric focusing) in adenocarcinoma of the uterine body.

作者信息

Horvath G, Fernö M, Tropé C

出版信息

Neoplasma. 1987;34(3):313-7.

PMID:3614465
Abstract

Thirty-two samples from adenocarcinoma of the uterine body were analyzed for estrogen receptor (ER). ER was measured with two techniques: isoelectric focusing (IF) in polyacrylamide gels and enzyme immuno assay (EIA) with monoclonal antibodies. When comparing, a very good correlation between IF and EIA was found. ER recovery was, however, increased twice when using the latter technique. The advantages of EIA in terms of its rapidity and simplicity should be considered against its, at present, greater expenses compared with methods based on radiolabeled ligands.

摘要

对32例子宫体腺癌样本进行了雌激素受体(ER)分析。采用两种技术检测ER:聚丙烯酰胺凝胶等电聚焦(IF)和单克隆抗体酶免疫测定(EIA)。比较时发现,IF与EIA之间具有很好的相关性。然而,使用后一种技术时,ER回收率提高了两倍。考虑到EIA在速度和简便性方面的优势,也要看到其目前与基于放射性标记配体的方法相比费用更高的问题。

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