Lucas-Heron B, Fontenaille C
Biochim Biophys Acta. 1979 May 17;553(2):284-94. doi: 10.1016/0005-2736(79)90232-3.
Urate transport in human erythrocytes were measured and compared to previous observations by other authors regarding inorganic anions, especially chloride. Conclusions wwere as follows: 1. Urate influx as a function of increasing concentrations showed saturation kinetics. 2. The effects of pH and of several passive anion transport inhibitors such as dinitrofluorobenzene, sodium salicylate, sodium benzoate and phenylbutazone suggest that urate and chloride are transported by different mechanisms. 3. Urate influx seems to depend on intracellular glycolysis. The results obtained on red blood cells after glycolysis inhibition agree with those obtained on ghosts where metabolism does not take place. 4. The large drop in urate influxes into erythrocytes in the presence of a glycolysis inhibitor and of a passive ion transport inhibitor seems to argue in favour of a dual urate transport mechanism, one for passive diffusion and the other connected with glycolysis. 5. The drop in the urate influx into ghosts in the absence of ATP suggests that the latter might intervene in urate transport by human red cell membranes.
对人类红细胞中的尿酸转运进行了测量，并与其他作者先前关于无机阴离子，尤其是氯离子的观察结果进行了比较。结论如下：1. 随着浓度增加，尿酸流入呈现饱和动力学。2. pH值以及几种被动阴离子转运抑制剂（如二硝基氟苯、水杨酸钠、苯甲酸钠和保泰松）的影响表明，尿酸和氯离子通过不同机制转运。3. 尿酸流入似乎依赖于细胞内糖酵解。糖酵解抑制后在红细胞上获得的结果与在无代谢的血影上获得的结果一致。4. 在存在糖酵解抑制剂和被动离子转运抑制剂的情况下，红细胞中尿酸流入大幅下降，这似乎支持了一种双重尿酸转运机制，一种用于被动扩散，另一种与糖酵解相关。5. 在无ATP的情况下，血影中尿酸流入下降，这表明ATP可能参与人类红细胞膜的尿酸转运。
