Chen Di, Chen Xiaomin, Xie Hua-Tao, Hatton Mark P, Liu Xiaowei, Liu Yang
Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Ophthalmology, Zhongnan Hospital, Wuhan University, Wuhan, China.
Front Med (Lausanne). 2022 Sep 6;9:981610. doi: 10.3389/fmed.2022.981610. eCollection 2022.
Extracellular matrix (ECM) is a key component of the stem cell local microenvironment. Our study aims to explore the periglandular distribution of major components of ECM in the Meibomian gland (MG).
Human eyelids and mouse eyelids were collected and processed for immunofluorescence staining.
Human MG tissues stained positive for collagen IV α1, collagen IV α2, collagen IV α5, and collagen IV α6 around the acini and duct, but negative for collagen IV α3 and collagen IV α4. The mouse MG were stained positive for the same collagen IV subunits as early as postnatal day 15. Laminin α2, laminin β1 and perlecan stained the regions surrounding the acini and the acinar/ductal junction in the human MG, but not the region around the duct. Tenascin-C was found specifically located at the junctions between the acini and the central ducts. Neither agrin nor endostatin was found in the human MG tissues.
The ECM expresses specific components in different regions around the MG, which may play a role in MG stem cell regulation, renewal, and regeneration.
细胞外基质（ECM）是干细胞局部微环境的关键组成部分。我们的研究旨在探讨睑板腺（MG）中ECM主要成分的腺周分布。
收集人眼睑和小鼠眼睑并进行免疫荧光染色处理。
人MG组织在腺泡和导管周围，IV型胶原α1、IV型胶原α2、IV型胶原α5和IV型胶原α6染色呈阳性，但IV型胶原α3和IV型胶原α4染色呈阴性。小鼠MG早在出生后第15天，相同的IV型胶原亚基染色就呈阳性。层粘连蛋白α2、层粘连蛋白β1和基底膜聚糖在人MG的腺泡周围区域以及腺泡/导管连接处染色，但在导管周围区域未染色。肌腱蛋白-C特异性定位于腺泡与中央导管之间的连接处。在人MG组织中未发现聚集蛋白和内皮抑素。
ECM在MG周围的不同区域表达特定成分，这可能在MG干细胞的调控、更新和再生中发挥作用。
