School of Computer Science, Reichman University (IDC Herzliya), Herzliya, Israel.
Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel.
Sci Rep. 2022 Sep 23;12(1):15835. doi: 10.1038/s41598-022-19984-x.
Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy-a novel concept for molecular profiling of solid tumors using molecular sampling with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor by permeabilization of the cell membrane, it facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its non tissue destructive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy breast tissue and reflect spatial tumor differential protein expression. E-biopsy provides a completely new molecular sampling modality for solid tumors molecular cartography, providing information that potentially enables more rapid and sensitive detection at lesser risk, as well as more precise personalized medicine.
切除组织活检是癌症治疗和精准医学的核心手段，但会给患者带来风险，并且不能充分广泛且真实地反映实体瘤的异质性。在这里，我们引入 e 活检——这是一种使用电穿孔进行分子采样对实体瘤进行分子分析的新概念。由于 e 活检通过细胞膜的通透性获取实体瘤的分子组成，因此它可以在不进行组织切除的情况下进行肿瘤诊断。此外，由于其非组织破坏性的特点，e 活检能够在同一程序的几个不同位置多次探测实体瘤，从而实现肿瘤分子异质性的空间分析。我们使用小鼠的 4T1 乳腺癌模型在体内演示了 e 活检，以评估其性能以及推断的空间差异蛋白表达。具体来说，我们表明，通过体内 e 活检获得的蛋白质组学谱可以区分肿瘤与健康的乳腺组织，并反映出空间肿瘤差异蛋白表达。E 活检为实体瘤分子图谱提供了一种全新的分子采样方式，提供了潜在的更快速、更敏感、风险更小的检测信息，以及更精确的个性化医疗信息。
