两种重组 Lpt 蛋白的结晶
Protein Crystallization of Two Recombinant Lpt Proteins.
机构信息
Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
Institute of Biophysics - CNR, Milan, Italy.
出版信息
Methods Mol Biol. 2022;2548:249-263. doi: 10.1007/978-1-0716-2581-1_15.
The prerequisite for 3D structure determination of macromolecules via X-ray crystallography is well-ordered, diffracting crystals. Here, we report the recombinant production, biophysical/biochemical protein sample characterization, and vapor diffusion sitting drop crystallization protocols for two lipopolysaccharide transport proteins: LptH from Pseudomonas aeruginosa (Pa-LptH) and an inactive LptC mutant (G153R) from Escherichia coli (EcLptCG153R).
通过 X 射线晶体学测定大分子的 3D 结构的前提条件是有序的、可衍射的晶体。在这里，我们报告了两种脂多糖转运蛋白的重组生产、生物物理/生化蛋白质样品表征以及蒸汽扩散坐滴结晶方案：铜绿假单胞菌（Pa-LptH）的 LptH 和大肠杆菌（EcLptCG153R）的无活性 LptC 突变体（G153R）。
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