Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, USA.
Methods Mol Biol. 2023;2571:169-175. doi: 10.1007/978-1-0716-2699-3_17.
Multiple sclerosis is a demyelinating disease of the central nervous system characterized by the loss of the myelin sheath-the nonconductive membrane surrounding neuronal axons. Demyelination interrupts neuronal transmission, which can impair neurological pathways and present a variety of neurological deficits. Prolonged demyelination can damage neuronal axons resulting in irreversible neuronal damage. Efforts have been made to identify agents that can promote remyelination. However, the assessment of remyelination that new therapies promote can be challenging. The method described in this chapter addresses this challenge by using isobaric C13-histidine as a tag for monitoring its incorporation into myelin proteins and thus monitoring the remyelination process.
多发性硬化症是一种中枢神经系统脱髓鞘疾病，其特征是髓鞘丢失——围绕神经元轴突的非传导性膜。脱髓鞘会中断神经元的传递，从而损害神经通路并导致多种神经功能缺陷。长期脱髓鞘会损害神经元轴突，导致不可逆转的神经元损伤。人们一直在努力寻找可以促进髓鞘再生的药物。然而，评估新疗法促进的髓鞘再生可能具有挑战性。本章中描述的方法通过使用等压 C13-组氨酸作为标记物来监测其掺入髓鞘蛋白，从而监测髓鞘再生过程，解决了这一挑战。
