Grupo de Espectrometria Analítica Aplicada, Faculdade de Química, Instituto de Ciências Exatas E Naturais, Universidade Federal Do Pará, Belém, Pará, 66075-110, Brazil.
Biol Trace Elem Res. 2023 Jul;201(7):3513-3519. doi: 10.1007/s12011-022-03409-x. Epub 2022 Sep 24.
A simple procedure for determination of Al, Cr, K, Mg, Mn, and Zn using diluted organic acid in the preparation of shrimp (Macrobrachium amazonicum) and crab samples (Ucides cordatus) was proposed in this study. Determinations were performed using microwave-induced plasma optical emission spectrometer (MIP OES). The contents of elements were evaluated after solubilization of samples in 50% formic acid (v v) and subsequent heating in bath with stirring and heating at 90 °C. The accuracy of the proposed procedure was assessed using certified fish protein reference material (DORM-4) and the recovery percentages ranged from 91 to 117%. Microwave-assisted acid decomposition was used for a comparison of results with the procedure proposed using diluted formic acid, and the values obtained for all analytes were statistically equal at 95% confidence level. Cr levels were below the limit of detection. Potassium (7917-19,644 mg kg), Mg (1319-5376 mg kg), and Zn (43-307 mg kg) were the most abundant elements in the crustacean species studied can be considered good sources of these constituents for human diet. The proposed procedure using diluted formic acid was considered simple and suitable to determine Al, Cr, K, Mg, Mn, and Zn concentrations in crustaceans using MIP OES.
本研究提出了一种使用稀释有机酸在制备虾（亚马逊沼虾）和蟹样品（红蟹）中测定 Al、Cr、K、Mg、Mn 和 Zn 的简单方法。使用微波诱导等离子体光学发射光谱仪（MIP OES）进行测定。将样品在 50%甲酸（vv）中溶解后，在搅拌浴中加热并在 90°C 下加热，然后评估元素的含量。使用经认证的鱼蛋白参考物质（DORM-4）评估所提出程序的准确性，回收率范围为 91%至 117%。微波辅助酸分解用于比较与使用稀释甲酸提出的程序的结果，所有分析物的值在 95%置信水平下统计学上相等。Cr 水平低于检测限。在所研究的甲壳类动物中，K（7917-19644mgkg）、Mg（1319-5376mgkg）和 Zn（43-307mgkg）是最丰富的元素，可被认为是人类饮食中这些成分的良好来源。使用稀释甲酸的所提出的方法被认为简单且适合使用 MIP OES 测定甲壳类动物中的 Al、Cr、K、Mg、Mn 和 Zn 浓度。
