Department of Histology (Tissue Engineering Group), Faculty of Medicine, University of Granada, Granada, Spain.
Instituto de Investigación Biosanitaria, Ibs.GRANADA, Granada, Spain.
Methods Mol Biol. 2023;2566:175-186. doi: 10.1007/978-1-0716-2675-7_14.
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in the histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix, cryoprotect, and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
脂质是一组具有不同性质的物质，其特点是可溶于有机溶剂而不溶于水。脂质可以作为不同组织和器官的正常成分存在，并且可以受到多种病理条件的影响。脂质的组织化学鉴定在组织病理学诊断和研究中起着重要作用，但成功的染色取决于组织的充分固定和处理。在这里，我们描述了用于冷冻或石蜡包埋组织中脂质组织化学鉴定的固定、冷冻保护和处理组织样本的方法。
