Carriel Víctor, Campos Fernando, Aneiros-Fernández José, Kiernan John A
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Avenida de la investigación n° 11, Torre A, 5ª planta, 18016, Granada, Spain.
Instituto de Investigación Biosanitaria Ibs, Granada, Spain.
Methods Mol Biol. 2017;1560:197-206. doi: 10.1007/978-1-4939-6788-9_14.
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
脂质是一类异质性物质，其特点是可溶于有机溶剂而不溶于水。脂质可作为不同组织和器官的正常成分被发现，并且会受到多种病理状况的影响。脂质的组织化学鉴定在组织病理学诊断和研究中发挥着重要作用，但成功的染色取决于组织的充分固定和处理。在此，我们描述了用于冷冻或石蜡包埋组织中脂质组织化学鉴定的组织样本固定和处理方法。
