埃及麻风病患者白细胞介素-17A：临床、遗传和生化研究。
Interleukin-17A in Egyptian leprosy patients: a clinical, genetic, and biochemical study.
机构信息
Dermatology, Andrology and STDs Department, Faculty of Medicine, Menoufia University, Egypt.
Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Egypt.
出版信息
An Bras Dermatol. 2022 Nov-Dec;97(6):735-741. doi: 10.1016/j.abd.2021.09.016. Epub 2022 Sep 21.
BACKGROUND
Leprosy represents a long-term communicable disease resulting from Mycobacterium leprae infection. IL-17A is one of the pro-inflammatory cytokines that protects humans against many fungal and bacterial pathogens.
OBJECTIVE
To investigate IL-17A (rs2275913) gene polymorphism and its circulating level in leprosy patients, and to correlate the detected results with different clinical aspects of leprosy in the investigated patients.
METHODS
60 patients with leprosy, and 29 age and sex-matched volunteers were investigated for IL-17A serum level and IL-17A single nucleotide polymorphism (SNP) by ELISA and RFLP-PCR respectively.
RESULTS
IL-17A serum level was significantly higher in leprosy patients than in controls (p=0.034), and in TL than LL (p=0.017). IL-17A (rs2275913 A/G) G allele and GG genotype were associated significantly with LL (p=0.005and 0.001 respectively). IL-17A (rs2275913 A/G) AG genotype carriers demonstrated the highest IL-17A serum levels; however, its lowest levels were found in IL-17A (rs2275913 A/G) AA genotype carriers (p=0.005). Grade 2 disability (p=0.030) and positive slit skin smear (SSS) (p=0.005) were significantly associated with IL-17A (rs2275913 A/G) GG genotype.
STUDY LIMITATIONS
The small number of studied subjects.
CONCLUSIONS
IL -17A may have a pivotal role in leprosy pathogenesis. IL-17A (rs2275913) GG genotype plus G allele might be related to the development of LL in the Egyptian population.
背景
麻风病是一种由麻风分枝杆菌感染引起的长期传染性疾病。白细胞介素 17A（IL-17A）是一种促炎细胞因子，可保护人体免受许多真菌和细菌病原体的侵害。
目的
研究白细胞介素 17A（rs2275913）基因多态性及其在麻风病患者中的循环水平，并将检测结果与患者不同的麻风临床特征相关联。
方法
通过酶联免疫吸附试验（ELISA）和限制性片段长度多态性聚合酶链反应（RFLP-PCR）分别检测 60 例麻风病患者和 29 名年龄和性别匹配的志愿者的血清白细胞介素 17A 水平和白细胞介素 17A 单核苷酸多态性（SNP）。
结果
麻风病患者的白细胞介素 17A 血清水平明显高于对照组（p=0.034），瘤型麻风（TT）患者明显高于界线类偏瘤型麻风（BL）患者（p=0.017）。白细胞介素 17A（rs2275913 A/G）G 等位基因和 GG 基因型与界线类偏瘤型麻风（BL）显著相关（p=0.005 和 0.001）。白细胞介素 17A（rs2275913 A/G）AG 基因型携带者的白细胞介素 17A 血清水平最高；然而，白细胞介素 17A（rs2275913 A/G）AA 基因型携带者的白细胞介素 17A 血清水平最低（p=0.005）。2 级残疾（p=0.030）和阳性皮肤划痕试验（SSS）（p=0.005）与白细胞介素 17A（rs2275913 A/G）GG 基因型显著相关。
研究局限性
研究对象数量较少。
结论
白细胞介素 17A 可能在麻风病发病机制中起关键作用。在埃及人群中，白细胞介素 17A（rs2275913）GG 基因型加 G 等位基因可能与界线类偏瘤型麻风的发生有关。
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