TNFAIP6 定义了具有增强免疫抑制活性的 MSC 亚群。
TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities.
机构信息
Department of Immunology, School of Medicine, Health Science Center, Shenzhen University, Nanhai Avenue 3688, Shenzhen, 518060, Guangdong, People's Republic of China.
Shenzhen Key Laboratory for Reproductive Immunology of Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, 518060, Guangdong, People's Republic of China.
出版信息
Stem Cell Res Ther. 2022 Sep 24;13(1):479. doi: 10.1186/s13287-022-03176-5.
BACKGROUND
Mesenchymal stromal/stem cells (MSCs) have been intensively investigated in both pre-clinical and clinical studies. However, the therapeutic efficacy varies resulting from the heterogenicity of MSCs. Therefore, purifying the specific MSC subpopulation with specialized function is necessary for their therapeutic applications.
METHODS
The large-scale RNA sequencing analysis was performed to identify potential cell markers for the mouse MSCs. Then, the immune suppression activities of the purified MSC subpopulation were assessed in vitro and in vivo.
RESULTS
The TNFAIP6 (tumor necrosis factor alpha-induced protein 6) has been identified as a potential cell marker for mouse MSCs, irrespective of tissue origin and laboratory origin. The TNFAIP6 mouse MSCs showed enhanced immune suppression activities and improved therapeutic effects on the mouse model of acute inflammation, resulting from faster response to immune stimulation.
CONCLUSIONS
Therefore, we have demonstrated that the TNFAIP6 MSC subpopulation has enhanced immune suppression capabilities.
背景
间充质基质/干细胞（MSCs）在临床前和临床研究中都得到了深入研究。然而，由于 MSCs 的异质性，其治疗效果各不相同。因此，对于其治疗应用，有必要纯化具有特定功能的特定 MSC 亚群。
方法
进行了大规模的 RNA 测序分析，以鉴定用于小鼠 MSCs 的潜在细胞标记物。然后，在体外和体内评估了纯化的 MSC 亚群的免疫抑制活性。
结果
无论组织来源和实验室来源如何，肿瘤坏死因子α诱导蛋白 6（TNFAIP6）已被鉴定为小鼠 MSCs 的潜在细胞标记物。与对照组相比，TNFAIP6 小鼠 MSCs 表现出增强的免疫抑制活性，并改善了对急性炎症小鼠模型的治疗效果，这是由于对免疫刺激的反应更快。
结论
因此，我们已经证明，TNFAIP6 MSC 亚群具有增强的免疫抑制能力。
Zotero 插件