Department of Obstetrics and Gynecology, Osaka Medical and Pharmaceutical University, 2-7 Daigakumachi, Takatsuki, Osaka, 569-8686, Japan.
Department of Obstetrics and Gynecology, Osaka Medical and Pharmaceutical University, 2-7 Daigakumachi, Takatsuki, Osaka, 569-8686, Japan.
Mol Cell Endocrinol. 2023 Jan 1;559:111779. doi: 10.1016/j.mce.2022.111779. Epub 2022 Sep 22.
MicroRNAs (miRNAs) are non-coding small RNA molecules that can be secreted into the circulation and which exist in remarkably stable forms. Circulating miRNAs regulate numerous biological process and are aberrantly expressed in pathological conditions. Differentially expressed circulating miRNAs have received attention as potential biomarkers for many diseases. In this study, we revealed that miR-515-5p was significantly upregulated in maternal serum from preeclampsia patients in comparison to normal pregnant women. Bioinformatics prediction and a dual-luciferase reporter gene assay revealed that miR-515-5p directly targets the X-linked inhibitor of apoptosis protein (XIAP) 3'-untranslated region. In addition, the overexpression of miR-515-5p inhibited the proliferation and invasion of HTR-8/SVneo trophoblast cells. The decreased XIAP expression and reduced epithelial-mesenchymal transition (EMT) were observed in the preeclamptic placenta. Collectively, miR-515-5p may play critical roles in the pathogenesis of preeclampsia through suppression of XIAP, and serum miR-515-5p may act as a potential biomarker for preeclampsia.
微小 RNA（miRNAs）是一类非编码的小 RNA 分子，可以分泌到循环系统中，并以高度稳定的形式存在。循环 miRNAs 调节着许多生物学过程，并在病理条件下异常表达。差异表达的循环 miRNAs 作为许多疾病的潜在生物标志物受到了关注。在这项研究中，我们发现与正常孕妇相比，子痫前期患者的母血清中 miR-515-5p 显著上调。生物信息学预测和双荧光素酶报告基因检测显示，miR-515-5p 可以直接靶向凋亡抑制因子（XIAP）的 3'UTR。此外，miR-515-5p 的过表达抑制了 HTR-8/SVneo 滋养层细胞的增殖和侵袭。子痫前期胎盘组织中观察到 XIAP 表达降低和上皮间质转化（EMT）减少。总之，miR-515-5p 通过抑制 XIAP 可能在子痫前期的发病机制中发挥重要作用，血清 miR-515-5p 可能作为子痫前期的潜在生物标志物。
