Collins F M, Carter P B
Infect Immun. 1978 Jul;21(1):41-7. doi: 10.1128/iai.21.1.41-47.1978.
Germfree mice were infected intragastrically, intravenously, or intraperitoneally with 10(3) to 10(9) viable Salmonella typhi Ty2, S. gallinarum 9240, or S. enteritidis 5694. The 50% lethal doses were compared with those for conventionally raised mice. Substantial growth of the salmonellae occurred in the intestinal tract of the germfree mice but, despite the presence of more than 10(9) viable S. typhi or S. gallinarum in the lumen, the liver and spleen cultures remained Salmonella-free, and all of the mice survived the oral challenge. The ileal and cecal Peyer's patches and the mesenteric lymph nodes of these mice contained 10(3) to 10(4) viable salmonellae within 24 h of introduction of the inoculum into the stomach. Despite this local involvement, the infection did not spread systemically even when host resistance was reduced by means of sublethal, whole-body gamma irradiation before oral challenge. Germfree mice infected orally with as few as 10 mouse-virulent S. enteritidis quickly developed severe diarrhea and died within 5 to 8 days as a result of a spreading systemic disease.
无菌小鼠通过胃内、静脉内或腹腔内途径感染10³至10⁹个活的伤寒沙门氏菌Ty2、鸡沙门氏菌9240或肠炎沙门氏菌5694。将50%致死剂量与常规饲养小鼠的50%致死剂量进行比较。沙门氏菌在无菌小鼠的肠道内大量生长，但是，尽管肠腔内存在超过10⁹个活的伤寒沙门氏菌或鸡沙门氏菌，肝脏和脾脏培养物仍未检出沙门氏菌，并且所有小鼠在口服攻击后均存活。在将接种物引入胃内24小时内，这些小鼠的回肠和盲肠派尔集合淋巴结以及肠系膜淋巴结含有10³至10⁴个活的沙门氏菌。尽管有这种局部感染，但即使在口服攻击前通过亚致死性全身γ射线照射降低宿主抵抗力，感染也未发生全身扩散。口服感染低至10个对小鼠有致病性的肠炎沙门氏菌的无菌小鼠很快出现严重腹泻，并因全身性疾病扩散在5至8天内死亡。
