Hohmann A W, Schmidt G, Rowley D
Infect Immun. 1978 Dec;22(3):763-70. doi: 10.1128/iai.22.3.763-770.1978.
Within 3 h after oral challenge of mice with Salmonella typhimurium, foci of infection developed in the Peyer's patches of the small intestine. The numbers of organisms in the cecum, although in excess of those found in the small intestine, were not firmly associated with the cecal wall but were present largely in the cecum's contents. The Peyer's patches at first were remarkably incapable of eliminating even small numbers of Salmonella, but at about 7 days after infection developed the ability to eliminate a less virulent strain of S. typhimurium. Selected strains of Salmonella of varied virulence, and hybrid Escherichia coli/Salmonella typhimurium with varied O-antigens, revealed that those of low virulence could multiply within the intestinal Peyer's patches at nearly the same rate as a virulent strain, and the ability to multiply within the Peyer's patches was not dependent upon O-antigen type or smooth lipopolysaccharide. The ability of these strains to adhere to intestinal mucosa in vitro did not reflect on their ability to colonize the Peyer's patches, although strains of high in vitro adhesive ability appeared in greater numbers initially after oral challenge. Anti-O serum, ineffective in reducing the in vitro adhesive ability of virulent S. typhimurium, when given with the oral challenge prevented Peyer's patch colonization but was unable to prevent the appearance of a systemic infection. Anti-H serum, although effective in vitro in preventing adherence, had no effect in vivo. These experiments suggest that adhesiveness is neither essential nor sufficient for the virulence of Salmonella and that the usual development of a systemic infection after colonization of the small intestinal Peyer's patches may be subverted by the presence of O-antibody.
用鼠伤寒沙门氏菌对小鼠进行口服攻击后3小时内，小肠派伊尔结出现感染灶。盲肠中的细菌数量虽然超过小肠中的数量，但并未牢固地附着于盲肠壁，而是主要存在于盲肠内容物中。起初，派伊尔结甚至无法清除少量的沙门氏菌，但在感染约7天后，其具备了清除毒力较低的鼠伤寒沙门氏菌菌株的能力。选择不同毒力的沙门氏菌菌株，以及具有不同O抗原的杂交大肠杆菌/鼠伤寒沙门氏菌，结果显示毒力低的菌株在肠道派伊尔结内的繁殖速度与毒力强的菌株几乎相同，且在派伊尔结内的繁殖能力并不取决于O抗原类型或光滑脂多糖。这些菌株在体外黏附肠黏膜的能力并不能反映它们在派伊尔结内定植的能力，尽管在口服攻击后，体外黏附能力强的菌株最初数量较多。抗O血清在降低毒力强的鼠伤寒沙门氏菌体外黏附能力方面无效，但在口服攻击时给予可防止派伊尔结定植，却无法防止全身性感染的出现。抗H血清虽然在体外能有效防止黏附，但在体内却没有效果。这些实验表明，黏附性对于沙门氏菌的毒力既非必要条件也非充分条件，并且小肠派伊尔结定植后通常出现的全身性感染可能会因O抗体的存在而被颠覆。
