Ma Xuehui, Kong Lingjing, Zhu Wen, Wang Yongli, Zhang Zhengbo, Tian Yaozhou
Department of Preclinical, Wuxi Hospital of Traditional Chinese Medicine, Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.
Department of Gastroenterology, Wuxi Hospital of Triditional Chinese Medcine, Wuxi, China.
Evid Based Complement Alternat Med. 2022 Sep 16;2022:3219001. doi: 10.1155/2022/3219001. eCollection 2022.
CAG is the most common precancerous disease of gastric cancer, which belongs to a kind of chronic gastritis. CAG is in close association with gastric cancer, which makes itself a critical node clinically in cancer prevention and treatment. Curcumol is a main active monomer in Fuzheng Huowei decoction, which has the properties of antioxidant, antiviral, and antitumor. In this study, the expression of SDF-1/CXCR4/NF-B was detected by in vivo and in vitro methods. Then, we found that the expressions of NF-B, SDF-1, CXCR4, and p-NF-B were decreased in the curcumol treatment group. Curcumol inhibited gastric cancer cells' viability, migration, and invasion and induced their apoptosis. After adding the lentivirus overexpressing SDF-1 to the curcumol treatment group, it was found that SDF-1, CXCR4, NF-B, and p-NF-B protein expressions were all increased, and the effect of curcumol on gastric cancer cells was reversed. In the nude mouse experiment, the tumor volume in the curcumol + SDF-1 group was the largest, and the tumor volume in the Fuzheng Huowei decoction + NC group was the smallest. In conclusion, curcumol effectively protects gastric tissue and inhibits the viability of gastric cancer cells, and curcumol regulates SDF-1/CXCR4/NF-B to play a therapeutic role in chronic atrophic gastritis and gastric cancer.
慢性萎缩性胃炎（CAG）是胃癌最常见的癌前疾病，属于慢性胃炎的一种。CAG与胃癌密切相关，使其成为临床上癌症防治的关键节点。莪术醇是扶正和胃方中的主要活性单体，具有抗氧化、抗病毒和抗肿瘤的特性。在本研究中，通过体内和体外方法检测SDF-1/CXCR4/NF-κB的表达。然后，我们发现莪术醇治疗组中NF-κB、SDF-1、CXCR4和p-NF-κB的表达均降低。莪术醇抑制胃癌细胞的活力、迁移和侵袭，并诱导其凋亡。在莪术醇治疗组中加入过表达SDF-1的慢病毒后，发现SDF-1、CXCR4、NF-κB和p-NF-κB蛋白表达均增加，且莪术醇对胃癌细胞的作用被逆转。在裸鼠实验中，莪术醇+SDF-1组的肿瘤体积最大，扶正和胃方+NC组的肿瘤体积最小。综上所述，莪术醇有效保护胃组织并抑制胃癌细胞的活力，且莪术醇通过调节SDF-1/CXCR4/NF-κB在慢性萎缩性胃炎和胃癌中发挥治疗作用。
