Tkacz Marta, Tarnowski Maciej, Poniewierska-Baran Agata, Serwin Karol, Madej-Michniewicz Anna, Deskur Anna, Czerny Bogusław, Starzyńska Teresa
Department of Physiology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.
Institute of Biology, University of Szczecin, 71-415 Szczecin, Poland.
Diagnostics (Basel). 2022 Mar 12;12(3):700. doi: 10.3390/diagnostics12030700.
(1) Background: stromal-derived factor-1 (SDF-1/CXCL12), hepatocyte and vascular-endothelial growth factors (HGF and VEGF) have been shown to facilitate cell motility, proliferation and promote local tumor progression and metastatic spread. Recent research shows the important role of these cytokines in gastric cancer (GC) progression. (2) Methods: 21 gastric cancer patients and 19 healthy controls were included in the study. SDF-1, HGF and VEGF levels were evaluated in sera by ELISA. Patients and control sera were used to stimulate CRL-1739 GC cell line, and chemotaxis, adhesion and proliferation potential were assessed. (3) Results: Concentrations of SDF-1, HGF and VEGF were significantly higher in patients than in controls. Chemotaxis and adhesion assays revealed a significant response of GC cells to patients' serum. Furthermore, significant relationships were seen between chemotactic/adhesion response and tumor stage. Serum from intestinal early GC patients produced significantly stronger chemotactic response when compared to patients with metastatic spread. In turn, serum from patients with distal metastases significantly increased the adhesion of GC cells when compared to sera from the patients with no distal metastases. We also observed that HGF strongly stimulated the proliferation of CRL-1739 cells. (4) Conclusions: We observed that the sera from GC patients, but also SDF-1, HGF and VEGF used alone, have a strong pro-metastatic effect on CRL-1739 cells. We also demonstrated that the concentration of these cytokines is specifically elevated in the sera of patients in an early stage of malignancy. Our results indicate that SDF-1, HGF and VEGF are very important molecules involved in gastric cancer progression.
（1）背景：基质细胞衍生因子-1（SDF-1/CXCL12）、肝细胞生长因子和血管内皮生长因子（HGF和VEGF）已被证明可促进细胞运动、增殖，并促进局部肿瘤进展和转移扩散。最近的研究表明这些细胞因子在胃癌（GC）进展中具有重要作用。（2）方法：本研究纳入21例胃癌患者和19例健康对照。通过酶联免疫吸附测定法（ELISA）评估血清中SDF-1、HGF和VEGF水平。使用患者和对照血清刺激CRL-1739胃癌细胞系，并评估趋化性、黏附性和增殖潜能。（3）结果：患者血清中SDF-1、HGF和VEGF的浓度显著高于对照组。趋化性和黏附性分析显示胃癌细胞对患者血清有显著反应。此外，趋化性/黏附性反应与肿瘤分期之间存在显著相关性。与有转移扩散的患者相比，肠道早期胃癌患者的血清产生的趋化反应明显更强。反过来，与无远处转移患者的血清相比，有远处转移患者的血清显著增加了胃癌细胞黏附。我们还观察到HGF强烈刺激CRL-1739细胞的增殖。（4）结论：我们观察到胃癌患者的血清以及单独使用的SDF-1、HGF和VEGF对CRL-1739细胞具有很强的促转移作用。我们还证明这些细胞因子的浓度在恶性肿瘤早期患者的血清中特异性升高。我们的结果表明SDF-1、HGF和VEGF是参与胃癌进展的非常重要的分子。
