Park Chang Ha, Park Ye Eun, Yeo Hyeon Ji, Yoon Jeong Su, Park Soo-Yun, Kim Jae Kwang, Park Sang Un
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Division of Life Sciences and Bio-Resource and Environmental Center, College of Life Sciences and Bioengineering, Incheon National University, Yeonsu-gu, Incheon 22012, Republic of Korea.
J Agric Food Chem. 2021 Feb 3;69(4):1300-1307. doi: 10.1021/acs.jafc.0c06863. Epub 2021 Jan 26.
We profiled and quantified primary (amine, organic acids, tricarboxylic acid cycle intermediates, amino acids, and carbohydrates) and secondary metabolites (triterpenoids, phenolic acids, carotenoids, flavonoids, and anthocyanins) in the edible parts (leaves and fruits) of the diploid and tetraploid cultivar L. 'Cheongil.' Through comprehensive metabolic profiling, the tetraploid mulberry cultivar was able to produce diverse metabolites supported by higher accumulation patterns of primary and secondary metabolites in their edible parts. In particular, the edible parts of the tetraploid showed higher accumulation patterns of most metabolites (amino acids, carbohydrates, carotenoids, and anthocyanins) than the diploid, which was supported by the results of principal component analyses (PCAs) showing a clear separation between the diploid and tetraploid groups. Additionally, this metabolome study comprehensively described the correlation between primary and secondary metabolites in the edible parts of diploid and tetraploid mulberry cultivars and provided information useful for plant breeding strategies to improve metabolite biosynthesis using polyploidy.
我们对二倍体和四倍体品种L. 'Cheongil'的可食用部分（叶片和果实）中的初级代谢物（胺类、有机酸、三羧酸循环中间体、氨基酸和碳水化合物）和次级代谢物（三萜类、酚酸、类胡萝卜素、黄酮类和花青素）进行了分析和定量。通过全面的代谢谱分析，四倍体桑树品种能够产生多种代谢物，这得益于其可食用部分中初级和次级代谢物的更高积累模式。特别是，四倍体的可食用部分在大多数代谢物（氨基酸、碳水化合物、类胡萝卜素和花青素）方面的积累模式高于二倍体，主成分分析（PCA）结果表明二倍体和四倍体组之间有明显区分，这支持了上述结论。此外，这项代谢组学研究全面描述了二倍体和四倍体桑树品种可食用部分中初级和次级代谢物之间的相关性，并为利用多倍体改善代谢物生物合成的植物育种策略提供了有用信息。
