冠状病毒基因组中 tRNA 样碱基序列的发现及其可能的作用机制。
Discovery of a tRNA-like base sequence in the coronavirus genome and possible mechanism of action.
机构信息
Yoshida Biological Laboratory Inc., 11-1, Takehanasotoda-cho, Yamashina-ku, Kyoto, 607-8081, Japan.
出版信息
Genes Genomics. 2022 Nov;44(11):1399-1404. doi: 10.1007/s13258-022-01299-w. Epub 2022 Sep 27.
BACKGROUND
The question of whether the coronavirus genome contain as-yetununderstood genetic component.
PURPOSE (OBJECTIVE): Elucidate the novel functions of the discovered tRNA-like base sequence and lead to the development of novel therapeutic agents.
METHODS
A novel tRNA-like base sequence was found in the sequences complementary to the genomes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and SARS-CoV. By comparing mutations in the tRNA-like base sequences of these two viruses, it was found that base pairing in the cloverleaf model of SARS-CoV-2 was more robust than that of SARS-CoV.
RESULTS
The results of homology search between a short sequence of the coronavirus tRNA-like base sequence and human genes suggest that the molecule produced by this novel tRNA-like sequence may be involved in the splicing of human messenger RNA.
CONCLUSIONS
Experimental molecular evidence of the tRNA-like base sequence discovered in this study is urgently needed.
背景
冠状病毒基因组是否包含尚未被理解的遗传成分这一问题。
目的（目标）：阐明新发现的 tRNA 样碱基序列的新功能，并开发新的治疗药物。
方法
在与严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）和 SARS-CoV 基因组互补的序列中发现了一种新型 tRNA 样碱基序列。通过比较这两种病毒的 tRNA 样碱基序列中的突变，发现 SARS-CoV-2 的三叶草模型中的碱基配对比 SARS-CoV 更稳定。
结果
冠状病毒 tRNA 样碱基序列的短序列与人类基因之间的同源性搜索结果表明，该新型 tRNA 样序列产生的分子可能参与人类信使 RNA 的剪接。
结论
本研究发现的 tRNA 样碱基序列的实验分子证据亟待获得。
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