Department of Biological Environment, Faculty of Bioresource Sciences, Akita Prefectural University, Shimo-Shinjyo Nakano, Akita, 010-0195, Japan.
Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo, 060-0810, Japan.
Arch Microbiol. 2022 Sep 27;204(10):647. doi: 10.1007/s00203-022-03261-6.
A novel sulfate-reducing bacterium, strain PPLL, was isolated from marsh soil. Cells of strain PPLL were rod-shaped with length of 1.5 μm and width of 0.7 μm. Growth was observed at 22-37 °C (optimum 35 °C) and pH 6.8-8.4 (optimum 7.3). Lactate, succinate, fumarate, formate and malate were utilized as electron donors for sulfate reduction. Fermentative growth was not observed on tested organic acids. Besides sulfate, sulfite, thiosulfate and elemental sulfur were utilized as electron acceptors. Hydrogen is used only in the presence acetate or yeast extract. The major fatty acid was C. The complete genome of strain PPLL was composed of a circular chromosome with length of 4.2 Mbp and G + C content of 57.7 mol%. Sequence analysis of the 16S rRNA gene showed that strain PPLL was affiliated with the genus Desulfofustis in the family Desulfocapsaceae. On the basis of differences in the phylogenetic and phenotypic properties between the strain and the type strain of the genus Desulfofustis, strain PPLL (DSM 110475 = JCM 39161) is proposed as the type strain of a new species, with name of Desulfofustis limnaeus sp. nov.
从沼泽土壤中分离到一株硫酸盐还原菌，菌株 PPLL。菌株 PPLL 的细胞呈杆状，长 1.5μm，宽 0.7μm。在 22-37°C（最佳 35°C）和 pH6.8-8.4（最佳 7.3）下观察到生长。乳酸盐、琥珀酸盐、富马酸盐、甲酸盐和苹果酸盐可用作硫酸盐还原的电子供体。在测试的有机酸上没有观察到发酵生长。除了硫酸盐、亚硫酸盐、硫代硫酸盐和元素硫外，还可以用作电子受体。只有在存在乙酸盐或酵母提取物的情况下才使用氢气。菌株 PPLL 的主要脂肪酸为 C。菌株 PPLL 的完整基因组由一个长度为 4.2 Mbp 的圆形染色体和 57.7 mol%的 G+C 含量组成。16S rRNA 基因序列分析表明，菌株 PPLL 属于脱硫梭菌科脱硫梭菌属。基于菌株与脱硫梭菌属的模式菌株在系统发育和表型特性上的差异，建议菌株 PPLL（DSM 110475=JCM 39161）作为一个新种的模式菌株，命名为 Limnaeus 脱硫梭菌 Desulfofustis limnaeus sp. nov.。
