Department of Biological Environment, Faculty of Bioresource Sciences, Akita Prefectural University. Shimo-Shinjyo Nakano, Akita, 010-0195, Japan.
The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo, 060-0819, Japan.
Arch Microbiol. 2022 Sep 22;204(10):640. doi: 10.1007/s00203-022-03259-0.
A novel sulfate-reducing bacterium, strain ASN36, was isolated from sediment of a brackish lake in Japan. Cells of strain ASN36 were not motile and rod-shaped, with length of 2.0-4.9 μm and width of 0.6-0.9 μm. Growth was observed at 5-35 °C with an optimum growth temperature of 25-30 °C. The pH range for growth was 6.6-8.8 with an optimum pH of 7.3. Major fatty acids were C ω7c and C. Under sulfate-reducing conditions, strain ASN36 utilized lactate, malate, pyruvate, butyrate, ethanol, butanol, glycerol, yeast extract and H/CO as growth substrate. Fermentative growth occurred on malate and pyruvate. The novel isolate used sulfate, sulfite and thiosulfate as electron acceptors. The genome of strain ASN36 is composed of a chromosome with length of 6.3 Mbp and G + C content of 55.1 mol%. Analyses of the 16S rRNA gene indicated that strain ASN36 is related to Desulfoluna species. Overall genome relatedness indices indicated that strain ASN36 does not belong to any existing species. In contrast to the closest relatives, strain ASN36 lacks genes for reductive dehalogenase required for organohalide respiration and does not use halogenated aromatics as electron acceptors. On the basis of its genomic and phenotypic properties, strain ASN36 (= DSM 111985 = JCM 39257) is proposed as the type strain of a new species, Desulfoluna limicola sp. nov.
从日本的一个半咸水湖中分离到一株新型硫酸盐还原菌，菌株 ASN36。菌株 ASN36 的细胞是不运动的杆状，长 2.0-4.9μm，宽 0.6-0.9μm。在 5-35°C 下生长，最适生长温度为 25-30°C。生长的 pH 范围为 6.6-8.8，最适 pH 为 7.3。主要脂肪酸为 C ω7c 和 C。在硫酸盐还原条件下，菌株 ASN36 利用乳酸盐、苹果酸盐、丙酮酸、丁酸盐、乙醇、正丁醇、甘油、酵母提取物和 H/CO 作为生长底物。在苹果酸盐和丙酮酸上发生发酵生长。新型分离株使用硫酸盐、亚硫酸盐和硫代硫酸盐作为电子受体。菌株 ASN36 的基因组由一个长度为 6.3 Mbp 的染色体和 55.1 mol%的 G+C 含量组成。16S rRNA 基因分析表明，菌株 ASN36 与 Desulfoluna 种有关。全基因组相关指数表明，菌株 ASN36 不属于任何现有物种。与最接近的亲缘关系相比，菌株 ASN36 缺乏进行有机卤化物呼吸所需的还原脱卤酶基因，并且不将卤代芳烃作为电子受体。基于其基因组和表型特性，菌株 ASN36（=DSM 111985=JCM 39257）被提议为一个新种 Desulfoluna limicola sp. nov.的模式菌株。
