Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Kanagawa 237-0061, Japan.
Int J Syst Evol Microbiol. 2022 Sep;72(9). doi: 10.1099/ijsem.0.005516.
A sulphate-reducing magnetotactic bacterium, designated strain FSS-1, was isolated from sediments and freshwater of Suwa Pond located in Hidaka, Saitama, Japan. Strain FSS-1 was a motile, Gram-negative and curved rod-shaped bacterium that synthesizes bullet-shaped magnetite (FeO) nanoparticles in each cell. Strain FSS-1 was able to grow in the range of pH 6.5-8.0 (optimum, pH 7.0), 22-34 °C (optimum, 28 °C) and with 0-8.0 g l NaCl (optimum, 0-2.0 g l NaCl). Strain FSS-1 grew well in the presence of 50 µM ferric quinate as an iron source. The major fatty acids were anteiso-C, iso-C and anteiso-C. The major menaquinone was MK-7 (H). Strain FSS-1 contained desulfoviridin, cytochrome and catalase, but did not contain oxidase. Strain FSS-1 used fumarate, lactate, pyruvate, malate, formate/acetate, succinate, tartrate, ethanol, 1-propanol, peptone, soytone and yeast extract as electron donors, while the strain used sulphate, thiosulphate and fumarate as electron acceptors. Fumarate was fermented in the absence of electron acceptors. Analysis of the 16S rRNA gene sequence showed that strain FSS-1 is a member of the genus . The gene sequence showed 96.7, 95.0, 92.0, 91.2 and 91.4% similarities to the most closely related members of the genera B7-43, BSY, DSM 107105, ThAc01 and RS-1, respectively. The DNA G+C content of strain FSS-1 was 67.5 mol%. The average nucleotide identity value between strain FSS-1 and B7-43 was 80.7 %. Therefore, strain FSS-1 represents a novel species within the genus , for which the name sp. nov. is proposed (=JCM 32405=DSM 110007).
从日本埼玉县Hideka的 Suwa 池塘的沉积物和淡水中分离到一株硫酸盐还原磁细菌，命名为 FSS-1 株。FSS-1 株是一种运动的革兰氏阴性弯曲杆状细菌，每个细胞中都合成子弹形磁铁矿（FeO）纳米颗粒。FSS-1 株能够在 pH 6.5-8.0（最佳，pH 7.0）、22-34°C（最佳，28°C）和 0-8.0 g l NaCl（最佳，0-2.0 g l NaCl）的范围内生长。FSS-1 株在 50 µM 奎宁酸铁作为铁源的存在下生长良好。主要脂肪酸为 anteiso-C、iso-C 和 anteiso-C。主要的menaquinone 是 MK-7（H）。FSS-1 株含有脱硫弧菌、细胞色素 c 和过氧化氢酶，但不含有氧化酶。FSS-1 株使用延胡索酸盐、乳酸盐、丙酮酸盐、苹果酸盐、甲酸盐/乙酸盐、琥珀酸盐、酒石酸盐、乙醇、1-丙醇、蛋白胨、大豆胨和酵母提取物作为电子供体，而该菌株则使用硫酸盐、硫代硫酸盐和延胡索酸盐作为电子受体。在没有电子受体的情况下，延胡索酸盐被发酵。16S rRNA 基因序列分析表明，FSS-1 株是属的一个成员。基因序列与属的最密切相关成员的相似度分别为 96.7%、95.0%、92.0%、91.2%和 91.4%，分别为 B7-43、BSY、DSM 107105、ThAc01 和 RS-1。FSS-1 株的 DNA G+C 含量为 67.5 mol%。FSS-1 株与 B7-43 株的平均核苷酸同一性值为 80.7%。因此，FSS-1 株代表属内的一个新种，提议命名为 sp. nov.（=JCM 32405=DSM 110007）。
