Departamento de Ciências Básicas, Universidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatuba, São Paulo, Brazil.
Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - SBFis, Universidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatuba, São Paulo, Brazil.
Arch Oral Biol. 2022 Nov;143:105551. doi: 10.1016/j.archoralbio.2022.105551. Epub 2022 Sep 21.
The study aimed to assess the effects of mate tea [Ilex paraguariensis] on the redox state and biochemical parameters of salivary glands in diabetic male rats.
Twenty-four male Wistar rats (3 months old) were randomly divided into groups (n = 8 per group): control rats that received water (C); diabetic rats that received water (D); diabetic rats treated with mate tea (DMT). The treated streptozotocin-induced diabetic rats were given mate tea powder by intragastric gavage at a dose of 20 mg/kg daily for 28 days. Content of total protein, amylase, oxidative lipid damage, measured as thiobarbituric acid reactive substances (TBARs), oxidative protein damage, measured as protein carbonyl, total antioxidant capacity, uric acid, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were examined by the spectrophotometric method in the parotid and submandibular glands.
The D group showed lower total protein, amylase, TBARs, protein carbonyl, total antioxidant capacity, GSH, uric acid, and GPx than the C group in both salivary glands, as well as higher SOD and CAT activities. The DMT group showed higher total protein, amylase, total antioxidant capacity, GSH, uric acid, and GPx than the D group in both salivary glands. Moreover, mate tea increased SOD in the parotid gland and CAT in the submandibular gland of diabetic rats but did not influence TBARs and protein carbonyl in either salivary gland compared to D group.
Mate tea increased tissue protein synthesis and improved antioxidant defenses in the salivary glands of streptozotocin-induced diabetic male rats.
本研究旨在评估马黛茶（Ilex paraguariensis）对糖尿病雄性大鼠唾液腺氧化还原状态和生化参数的影响。
将 24 只 3 月龄雄性 Wistar 大鼠随机分为 4 组（每组 8 只）：对照组大鼠给予水（C）；糖尿病组大鼠给予水（D）；马黛茶治疗糖尿病组大鼠（DMT）。用链脲佐菌素诱导糖尿病大鼠，每天通过灌胃给予 20mg/kg 的马黛茶粉末，共 28 天。用分光光度法检测腮腺和颌下腺中总蛋白、淀粉酶、氧化脂质损伤（用硫代巴比妥酸反应物质（TBARs）表示）、氧化蛋白损伤（用蛋白羰基表示）、总抗氧化能力、尿酸、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的含量。
与 C 组相比，D 组在两种唾液腺中总蛋白、淀粉酶、TBARs、蛋白羰基、总抗氧化能力、GSH、尿酸和 GPx 水平均较低，SOD 和 CAT 活性较高。与 D 组相比，DMT 组在两种唾液腺中总蛋白、淀粉酶、总抗氧化能力、GSH、尿酸和 GPx 水平均较高。此外，与 D 组相比，马黛茶增加了糖尿病大鼠腮腺中的 SOD 和颌下腺中的 CAT，但对两种唾液腺中的 TBARs 和蛋白羰基均无影响。
马黛茶增加了组织蛋白合成，并改善了链脲佐菌素诱导的糖尿病雄性大鼠唾液腺的抗氧化防御能力。
