Universidade de São Paulo - USP, School of Dentistry, Department of Biomaterials and Oral Biology, São Paulo, SP, Brazil.
Universidade Estadual de Campinas - Unicamp, Institute of Biology, Department of Plant Biology, Campinas, SP, Brazil.
Braz Oral Res. 2022 Mar 14;36:e034. doi: 10.1590/1807-3107bor-2022.vol36.0034. eCollection 2022.
This study aimed to analyze oxidative stress and the activity of antioxidant enzymes in the salivary glands of streptozotocin (STZ)-induced diabetic rats with ad libitum consumption of chamomile tea in substitution of water for 21 days. Rats were divided in two control groups (untreated control and treated control) and two diabetic groups (untreated diabetic and treated diabetic). Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, total antioxidant status (TAS), and malondialdehyde (MDA) concentrations were determined. The chemical composition of the chamomile essential oil revealed 39 compounds, accounting for 93.5% of the total oils. The polyphenolic profile of the tea showed the presence of apigenin, luteolin, umbelliferone, and esculetin. SOD, GPx, CAT, and TAS levels were lower in the parotid (PA) diabetic glands, but treatment increased their concentration in both the submandibular (SM) and PA diabetic salivary glands. Increased MDA levels were observed in the PA diabetic glands, which were decreased by the consumption of chamomile tea with a reduction in hyperglycemia compared to that in untreated diabetic rats. However, the SM diabetic glands showed no difference in the MDA content. The consumption of chamomile tea prevented oxidative stress in the PA glands of diabetic rats, exhibiting hypoglycemic and antioxidant effects. Thus, chamomile tea could be a potential candidate for preventing oral complications in diabetes mellitus.
本研究旨在分析自由摄取菊苣茶替代水 21 天后，链脲佐菌素（STZ）诱导的糖尿病大鼠唾液腺中的氧化应激和抗氧化酶活性。大鼠分为两组对照（未处理对照和处理对照）和两组糖尿病组（未处理糖尿病组和处理糖尿病组）。测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）活性、总抗氧化状态（TAS）和丙二醛（MDA）浓度。菊苣精油的化学成分显示 39 种化合物，占总油量的 93.5%。茶的多酚谱显示存在芹菜素、木樨草素、茴芹素和七叶苷。糖尿病大鼠腮腺（PA）中的 SOD、GPx、CAT 和 TAS 水平较低，但治疗后可增加下颌下腺（SM）和 PA 糖尿病唾液腺中的浓度。PA 糖尿病腺体中的 MDA 水平升高，与未治疗的糖尿病大鼠相比，菊苣茶的消耗降低了血糖水平，从而降低了 MDA 水平。然而，SM 糖尿病腺体中的 MDA 含量没有差异。菊苣茶的消耗可预防糖尿病大鼠 PA 腺体中的氧化应激，表现出降血糖和抗氧化作用。因此，菊苣茶可能是预防糖尿病口腔并发症的潜在候选药物。
