Lopez Arrieta Zulieth, Rodríguez-Cavallo Erika, Méndez-Cuadro Darío
Faculty Health and Medical Science, University of Sinú, Cartagena 130014, Colombia.
Analytical Chemistry and Biomedicine Group, Exacts and Natural Sciences Faculty, University of Cartagena, Cartagena 130014, Colombia.
Dent J (Basel). 2024 Jul 8;12(7):208. doi: 10.3390/dj12070208.
Gingival enlargement is a common clinical sign in the gingival diseases associated with orthodontic treatment. Its biological mechanisms are not completely understood; nevertheless, the biochemical changes associated with these inflammatory and overgrowth processes could alter the post-translational protein modifications occurring in various locations within the mouth. Here, changes in the profiles of the carbonylated and phosphorylated proteins in saliva were examined in donors with gingival enlargement (seven men and seven women) and healthy donors (six men and eight women). The sociodemographic characteristics of both groups did not present significant differences. Carbonylation was measured by a quantitative immunoassay (Dot Blot), whereas the profiles of the phosphorylated proteins were visualized by SDS-PAGE with quercetin staining. Some phosphopeptides were also identified using a typical LC-MS-MS approach. Our results showed that gingival enlargement induced a significant increase in oxidative damage in salivary proteins. While a significant reduction in phosphorylation was observed at the stain level in SDS-PAGE, there was a slight increase in the number of phosphorylated proteins identified by MS in samples with gingival enlargement.
牙龈肿大是正畸治疗相关牙龈疾病中的常见临床体征。其生物学机制尚未完全明确；然而，与这些炎症和过度生长过程相关的生化变化可能会改变口腔内不同部位发生的蛋白质翻译后修饰。在此，对牙龈肿大的捐赠者（7名男性和7名女性）和健康捐赠者（6名男性和8名女性）唾液中羰基化和磷酸化蛋白质的谱图变化进行了检测。两组的社会人口统计学特征没有显著差异。通过定量免疫测定法（斑点印迹法）测量羰基化，而通过槲皮素染色的SDS-PAGE观察磷酸化蛋白质的谱图。还使用典型的LC-MS-MS方法鉴定了一些磷酸肽。我们的结果表明，牙龈肿大导致唾液蛋白质氧化损伤显著增加。虽然在SDS-PAGE染色水平观察到磷酸化显著减少，但在牙龈肿大样本中，通过质谱鉴定的磷酸化蛋白质数量略有增加。
