College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
Open Biol. 2022 Sep;12(9):220108. doi: 10.1098/rsob.220108. Epub 2022 Sep 28.
In animals, starvation can increase the level of reactive oxygen species (ROS) in some tissues. Mitochondrial DNA (mtDNA) is more vulnerable to being attacked by ROS due to the lack of histone protection, leading to oxidative damage. However, whether starvation is associated with the genetic diversity of mtDNA remains unclear. Here, by using adult individuals of under three different feeding treatments (starvation, with the provision of only water, and normal feeding), based on the high-throughput sequencing results of the PCR amplicons of the partial sequences of the mitochondrial gene cytochrome c oxidase subunit I (), no significant difference in the mean number of mitochondrial haplotypes and the mean genetic distance of haplotypes within individuals were identified between the three treatment groups. Coupled with the low proportion of heterogeneous sequences within each individual, it suggested that starvation had a limited impact on mitotype genetic diversity and mitochondrial function. Nevertheless, starvation could significantly increase the sequence number of haplotypes containing specific mutations, and for males with higher levels of mitochondrial heteroplasmy than females in the normal feeding group, starvation could further increase their mitochondrial heteroplasmy.
在动物中，饥饿会导致某些组织中活性氧（ROS）水平升高。由于缺乏组蛋白的保护，线粒体 DNA（mtDNA）更容易受到 ROS 的攻击，从而导致氧化损伤。然而，饥饿是否与 mtDNA 的遗传多样性有关尚不清楚。在这里，我们通过对处于三种不同喂养处理（饥饿、仅提供水和正常喂养）下的 个体的成年个体进行研究，基于线粒体基因细胞色素 c 氧化酶亚基 I（）部分序列 PCR 扩增子的高通量测序结果，发现这三种处理组之间个体内线粒体单倍型的平均数量和单倍型的平均遗传距离没有显著差异。再加上每个个体内异质 序列的比例较低，这表明饥饿对 mitotype 遗传多样性和线粒体功能的影响有限。然而，饥饿可以显著增加含有特定突变的单倍型的序列数量，并且对于正常喂养组中雄性比雌性具有更高水平的线粒体异质性的个体，饥饿可以进一步增加其线粒体异质性。
