Liang Jianhe, Sun Xu, Yi Lei, Lv Jinyuan
Department of Plastic Burn and Cosmetic Center, The First Affiliated Hospital of Jiamusi University, Jiamusi, 154002, China.
Department of Burn, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Aesthetic Plast Surg. 2023 Feb;47(1):423-429. doi: 10.1007/s00266-022-03096-y. Epub 2022 Sep 27.
To investigate whether hyperbaric oxygen therapy can improve the survival rate of fat transplantation and analyze the possible mechanisms.
Ninety SD rats were randomly divided into 3 groups. All the rats were cut into pieces with about 5 mL of fat from the abdominal cavity, rinsed with normal saline for 3 times, and cleaned with cotton pad adsorption method. Then, 3 ml was removed, divided into 3 parts, and injected into three adjacent but not touching parts of the back. Group A received 1h/d hyperbaric oxygen therapy, group B received 2 h/d hyperbaric oxygen therapy, and group C received no hyperbaric oxygen therapy. The hyperbaric oxygen therapy lasted for 10 consecutive days. Fat grafts from one site were randomly removed at 2, 4, and 6 weeks after surgery, respectively. ① the survival rate of fat transplantation in three groups was compared. ② observe the pathological section; ③ immunohistochemistry was used to detect and compare the expression of vascular endothelial growth factor.
The survival rate of fat transplantation in group A was the highest. After subcutaneous transplantation of 1 ml of fat and 1 hour/day of continuous hyperbaric oxygen treatment for 10 days, the fat survival rates were 0.796 ± 0.071 ml, 0.644 ± 0.151 ml, and 0.473 ± 0.127 ml at the second, fourth, and sixth weeks, respectively. The survival rate of fat transplantation in group B was the second. After subcutaneous transplantation of 1 ml of fat and 2 hour/day of continuous hyperbaric oxygen treatment for 10 days, the survival rate of fat was 0.624 ± 0.220 ml, 0.494 ± 0.125 ml, and 0.329 ± 0.153 ml at the second, fourth, and sixth weeks, respectively. The survival rate of fat transplantation in group C was the lowest. After subcutaneous transplantation of 1 ml of fat and no hyperbaric oxygen treatment for 10 days, the fat survival rates were 0.461 ± 0.132 ml, 0.290 ± 0.112 ml and 0.169 ± 0.091 ml at the second, fourth, and sixth weeks, respectively. We have made changes in the abstract of the article and marked in red color.
Hyperbaric oxygen therapy is conducive to the survival of transplanted fat. Importantly, a short period of hyperbaric oxygen therapy (1 h/d) can promote the survival of transplanted fat.
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探讨高压氧治疗能否提高脂肪移植的成活率并分析其可能机制。
将90只SD大鼠随机分为3组。所有大鼠均取约5 mL腹腔脂肪切碎，用生理盐水冲洗3次，采用棉垫吸附法清理。然后取出3 mL，分为3份，分别注射到背部三个相邻但不相连的部位。A组每天接受1小时高压氧治疗，B组每天接受2小时高压氧治疗，C组不接受高压氧治疗。高压氧治疗连续进行10天。分别在术后2、4、6周随机取出一处的脂肪移植物。①比较三组脂肪移植的成活率。②观察病理切片；③采用免疫组化法检测并比较血管内皮生长因子的表达。
A组脂肪移植成活率最高。皮下移植1 mL脂肪并每天连续高压氧治疗1小时共10天后，在第2、4、6周时脂肪存活量分别为0.796±0.071 mL、0.644±0.151 mL、0.473±0.127 mL。B组脂肪移植成活率次之。皮下移植1 mL脂肪并每天连续高压氧治疗2小时共10天后，在第2、4、6周时脂肪存活率分别为0.624±0.220 mL、0.494±0.125 mL、0.329±0.153 mL。C组脂肪移植成活率最低。皮下移植1 mL脂肪且不进行高压氧治疗10天后，在第2、4、6周时脂肪存活量分别为0.461±0.132 mL、0.290±0.112 mL、0.169±0.091 mL。我们已对文章摘要进行了修改并用红色标注。
高压氧治疗有利于移植脂肪存活。重要的是，短期高压氧治疗（每天1小时）可促进移植脂肪存活。
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