Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, 130 Dongdeokro, Jung-gu, Daegu, 41944, South Korea.
V Plastic Clinics, Daegu, 41944, South Korea.
Aesthetic Plast Surg. 2019 Apr;43(2):498-513. doi: 10.1007/s00266-018-01304-2. Epub 2019 Jan 11.
To overcome unpredictable fat graft resorption, cell-assisted lipotransfer using stromal vascular fraction (SVF) has been introduced. However, its effect on cancer growth stimulation and its oncological safety are debatable. We investigated the effect of SVF on adjacent breast cancer and transplanted fat in a mouse model.
A breast cancer xenograft model was constructed by injecting 2 × 10 MDA-MB-231-luc breast cancer cells into the right lower back of 40 NOD/SCID mice. Two weeks later, cancer size was sorted according to signal density using an in vivo optical imaging system, and 36 mice were included. Human fat was extracted from the abdomen, and SVFs were isolated using a component isolator. The mice were divided into four groups: A, controls; B, injected with 30 μl SVF; C, injected with 0.5 ml fat and 30 μl saline; group D, injected with 0.5 ml fat and 30 μl SVF. Magnetic resonance imaging and three-dimensional micro-computed tomography volumetric analysis were performed at 4 and 8 weeks.
Tumor volume was 43.6, 42.3, 48.7, and 42.4 mm at the initial time point and 6780, 5940, 6080, and 5570 mm at 8 weeks in groups A, B, C, and D, respectively. Fat graft survival volume after 8 weeks was 49.32% and 62.03% in groups C and D, respectively. At 2-month follow-up after fat grafting in the xenograft model, SVF injection showed an increased fat survival rate and did not increase the adjacent tumor growth significantly.
Fat grafting with SVF yields satisfactory outcome in patients who undergo breast reconstruction surgery.
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为了克服不可预测的脂肪移植物吸收，使用基质血管成分 (SVF) 的细胞辅助脂肪转移已经被引入。然而，它对癌症生长刺激的影响及其肿瘤学安全性是有争议的。我们在小鼠模型中研究了 SVF 对邻近乳腺癌和移植脂肪的影响。
通过将 2×10 MDA-MB-231-luc 乳腺癌细胞注射到 40 只 NOD/SCID 小鼠的右下背部，构建乳腺癌异种移植模型。2 周后，使用体内光学成像系统根据信号密度对癌症大小进行分类，共纳入 36 只小鼠。从腹部提取人脂肪，并使用成分分离仪分离 SVF。将小鼠分为四组：A 组为对照组；B 组注射 30μl SVF；C 组注射 0.5ml 脂肪和 30μl 生理盐水；D 组注射 0.5ml 脂肪和 30μl SVF。在第 4 周和第 8 周进行磁共振成像和三维微计算机断层扫描体积分析。
在初始时间点，A、B、C 和 D 组的肿瘤体积分别为 43.6、42.3、48.7 和 42.4mm，8 周时分别为 6780、5940、6080 和 5570mm。第 8 周时，C 组和 D 组的脂肪移植物存活率分别为 49.32%和 62.03%。在异种移植模型中脂肪移植后的 2 个月随访中，SVF 注射显示出增加的脂肪存活率，并且不会显著增加邻近肿瘤的生长。
SVF 辅助脂肪移植在接受乳房重建手术的患者中取得了令人满意的效果。
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