State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Animal Model Exp Med. 2022 Oct;5(5):445-452. doi: 10.1002/ame2.12272. Epub 2022 Sep 27.
To understand the relationship between myocardial contractility and external stimuli, detecting ex vivo myocardial contractility is necessary.
We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, voltages, and calcium concentrations.
The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, contractility decreased with an increase in frequency (0.45 ± 0.11-0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradually increased as the voltage increased from 6 to 10 V (0.14 ± 0.02-0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17-1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN).
Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
为了理解心肌收缩性与外部刺激之间的关系，有必要检测离体心肌的收缩性。
我们阐述了一种使用 Myostation-Intact 系统在不同频率、电压和钙离子浓度下检测分离的 C57 小鼠乳头肌收缩性的方法。
结果表明，在 10V、500ms 脉冲持续时间和 1Hz 时，乳头肌的基础收缩力为 0.27±0.03mN。从 0.1 到 1.0Hz，收缩力随频率增加而降低（0.45±0.11-0.10±0.02mN）。电压引发的肌肉收缩力在 3 到 6V 之间变化，随着电压从 6 增加到 10V，收缩力逐渐增加（0.14±0.02-0.28±0.03mN）。此外，当钙离子浓度从 1.5 增加到 3mM 时，肌肉收缩力增加（0.45±0.17-1.11±0.05mN）；然而，当浓度增加到 7.5mM 时，收缩力停止增加（1.02±0.23mN）。
我们的方法保证了乳头肌在体外的存活率，并为 Myostation-Intact 用户提供了关于分离肌肉收缩性研究的指导。
