Department of Biotechnology, Institute of Graduate studies and Research, Alexandria University, Alexandria 21526, Egypt.
Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Int J Biol Macromol. 2022 Dec 1;222(Pt A):701-714. doi: 10.1016/j.ijbiomac.2022.09.200. Epub 2022 Sep 25.
Wound healing is a complicated cellular process with overlapping phases. Naringin (NAR); a flavanone glycoside, possesses numerous pharmacological effects such as anti-inflammatory, antioxidant and anti-apoptotic effects. In the current study, Arabic gum (AG)/pectin hydrogel was utilized to encapsulate NAR. Drug-loaded AG/pectin hydrogel exhibited excellent EE% of about 99.88 ± 0.096 and high DL% of about 16.64 ± 0.013. The formulated drug-loaded hydrogel was characterized using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and Zetasizer analyzer, besides determination of equilibrium degree of swelling (EDS%). Afterwards, wound healing potential of NAR-loaded AG/pectin hydrogel was evaluated in an in vivo animal model. Results manifested that NAR-loaded AG/pectin hydrogel was able to accelerate wound healing in terms of enhanced angiogenesis, re-epithelialization and collagen deposition. Furthermore, it significantly (P < 0.001) down-regulated the mRNA expression of inflammatory mediators (TNF-α) and apoptosis (BAX). In addition, NAR-loaded AG/pectin hydrogel was found to possess potent antioxidant activity as it enhanced the levels of SOD and GSH, besides decreasing the levels of MPO, MDA and nitrite. These data suggest that NAR-loaded AG/pectin hydrogel could be utilized in wound healing applications.
伤口愈合是一个复杂的细胞过程，具有重叠的阶段。柚皮苷（NAR）；一种类黄酮糖苷，具有许多药理作用，如抗炎、抗氧化和抗凋亡作用。在目前的研究中，阿拉伯树胶（AG）/果胶水凝胶被用于包裹 NAR。载药 AG/果胶水凝胶表现出优异的 EE%约为 99.88±0.096 和高 DL%约为 16.64±0.013。通过傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、扫描电子显微镜（SEM）和 Zetasizer 分析仪对载药水凝胶进行了表征，同时测定了平衡溶胀度（EDS%）。随后，在体内动物模型中评价了载 NAR 的 AG/果胶水凝胶的伤口愈合潜力。结果表明，载 NAR 的 AG/果胶水凝胶能够通过促进血管生成、再上皮化和胶原沉积来加速伤口愈合。此外，它显著（P<0.001）下调了炎症介质（TNF-α）和凋亡（BAX）的 mRNA 表达。此外，载 NAR 的 AG/果胶水凝胶被发现具有很强的抗氧化活性，因为它增加了 SOD 和 GSH 的水平，同时降低了 MPO、MDA 和亚硝酸盐的水平。这些数据表明，载 NAR 的 AG/果胶水凝胶可用于伤口愈合应用。
