Marine College, Shandong University, Weihai, 264209, Shandong, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China.
Arch Microbiol. 2021 Nov;203(9):5397-5403. doi: 10.1007/s00203-021-02524-y. Epub 2021 Aug 16.
A bacterial strain, designated M625, was isolated from the surface of a marine red alga. Phylogenetic trees were reconstructed based on the 16S rRNA gene and RpoB protein sequences, which indicated that the strain belongs to the genus Aquimarina within the family Flavobacteriaceae. Strain M625 showed high sequence similarities to A. aggregata RZW4-3-2 (95.7%), A. seongsanensis CBA3208 (95.3%) and A. versatilis CBA3207 (95.0%). The AAI and POCP values between strain M625 and A. muelleri DSM 19832 were 71.8% and 57.9% respectively. The dDDH and ANI values between strain M625 and A. aggregata were 19.5% and 74.6% respectively. The strain was Gram-stain negative, strictly aerobic, non-motile and long rod-shaped, and positive for hydrolysis of starch, cellulose, alginate, DNA and Tween 20. The dominant respiratory quinone was MK-6. The major fatty acids were iso-C, iso-C 3-OH, and iso-C G, and the polar lipids consisted of phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids, and seven unidentified lipids. Based on the polyphasic comparisons, strain M625 is proposed to represent a novel species within the genus Aquimarina, for which the name Aquimarina algicola sp. nov. (type strain M625 = MCCC 1H00399 = KCTC 72685) was proposed.
一株细菌菌株，命名为 M625，从海洋红藻的表面分离得到。基于 16S rRNA 基因和 RpoB 蛋白序列构建的系统发育树表明，该菌株属于黄杆菌科 Aquimarina 属。菌株 M625 与 A. aggregata RZW4-3-2（95.7%）、A. seongsanensis CBA3208（95.3%）和 A. versatilis CBA3207（95.0%）具有高的序列相似性。菌株 M625 与 A. muelleri DSM 19832 的 AAI 和 POCP 值分别为 71.8%和 57.9%。菌株 M625 与 A. aggregata 的 dDDH 和 ANI 值分别为 19.5%和 74.6%。该菌株革兰氏染色阴性，严格需氧，不运动，呈长杆状，可水解淀粉、纤维素、褐藻胶、DNA 和 Tween 20。主要的呼吸醌是 MK-6。主要脂肪酸为 iso-C、iso-C 3-OH 和 iso-C G，极性脂类由磷脂酰乙醇胺、一种未鉴定的磷脂、两种未鉴定的胺脂和七种未鉴定的脂类组成。基于多相分类比较，菌株 M625 代表 Aquimarina 属中的一个新种，建议命名为Algicola aquimarina sp. nov.（模式菌株 M625= MCCC 1H00399= KCTC 72685）。
