利用 dCas9-SunTag 平台调控基因表达。

Regulation of Gene Expression Using dCas9-SunTag Platforms.

机构信息

Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan.

出版信息

Methods Mol Biol. 2023;2577:189-195. doi: 10.1007/978-1-0716-2724-2_13.

DOI:10.1007/978-1-0716-2724-2_13

PMID:36173574

Abstract

Regulating gene expression is important for basic research and therapeutic applications. The epigenome is a record of genetic modifications such as DNA methylation and histone modifications, and epigenetic changes can play a key role in modifying gene expression. With the advent of genome editing technologies, it has become possible to manipulate the epigenome of specific genomic regions to control gene expression. In particular, CRISPR-Cas9 systems have been used widely for epigenome editing due to their high efficiency, versatility, specificity, and ease of use. Here, we describe a protocol for the upregulation of specific genes using the dCas9-SunTag system.

摘要

调控基因表达对于基础研究和治疗应用都很重要。表观基因组是遗传修饰的记录，如 DNA 甲基化和组蛋白修饰，表观遗传变化可以在修饰基因表达中发挥关键作用。随着基因组编辑技术的出现，已经有可能操纵特定基因组区域的表观基因组来控制基因表达。特别是，由于其高效率、多功能性、特异性和易用性，CRISPR-Cas9 系统已被广泛用于表观基因组编辑。在这里，我们描述了一种使用 dCas9-SunTag 系统上调特定基因的方案。