Sharma R K, Singh K, Saxena K K
Vet Parasitol. 1987 May;24(3-4):211-20. doi: 10.1016/0304-4017(87)90042-2.
Adult Ascaridia galli and Heterakis gallinae obtained from the fowl (Gallus gallus) were treated in vitro with 10(-2) to 10(-5) M parbendazole and piperazine adipate for 10-60 min at 38 degrees C. Both the compounds at 10(-2) M caused mortality of A. galli and H. gallinae after a maximum of 30 min exposure. The effect of the drugs on the homogenates of the treated worm was investigated. Parbendazole (10(-2) M) inhibited malate oxidation by 68% in A. galli and 62% in H. gallinae. Piperazine adipate (10(-2) M) inhibited malate oxidation by 78% in both parasites. In A. galli oxaloacetate reduction was inhibited by 41 and 26% by 10(-2) M parbendazole and piperazine adipate, respectively; with H. gallinae this inhibition was found to be 39 and 55%, respectively. Aldolase activity in both the parasites was also inhibited by 10(-2) M parbendazole and piperazine adipate. Both compounds caused an inhibition of acid phosphomonoesterase activity, but the activities of lactate dehydrogenase and alkaline phosphomonoesterase were not affected significantly. Parbendazole (10(-2) M) had no significant effect on the cholinesterase activity of these parasites, but piperazine adipate (10(-2) M) caused an inhibition of 96% in A. galli and 93% in H. gallinae. The possible mode of action of the drugs is discussed.
从家禽（家鸡）体内获取的成年鸡蛔虫和鸡异刺线虫，在38℃下用10⁻²至10⁻⁵M的丙硫苯咪唑和己二酸哌嗪进行体外处理10 - 60分钟。两种化合物在10⁻²M浓度下，暴露最多30分钟后均可导致鸡蛔虫和鸡异刺线虫死亡。研究了药物对经处理虫体匀浆的影响。丙硫苯咪唑（10⁻²M）在鸡蛔虫中抑制苹果酸氧化68%，在鸡异刺线虫中抑制62%。己二酸哌嗪（10⁻²M）在两种寄生虫中均抑制苹果酸氧化78%。在鸡蛔虫中，10⁻²M丙硫苯咪唑和己二酸哌嗪分别抑制草酰乙酸还原41%和26%；在鸡异刺线虫中，这种抑制分别为39%和55%。10⁻²M丙硫苯咪唑和己二酸哌嗪也抑制了两种寄生虫的醛缩酶活性。两种化合物均导致酸性磷酸单酯酶活性受到抑制，但乳酸脱氢酶和碱性磷酸单酯酶的活性未受到显著影响。丙硫苯咪唑（10⁻²M）对这些寄生虫的胆碱酯酶活性无显著影响，但己二酸哌嗪（10⁻²M）在鸡蛔虫中导致96%的抑制，在鸡异刺线虫中导致93%的抑制。讨论了药物可能的作用方式。
