College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
Food Chem. 2023 Feb 15;402:134357. doi: 10.1016/j.foodchem.2022.134357. Epub 2022 Sep 21.
We have proposed a novel electrochemical aptasensing strategy for efficiently detecting staphylococcus aureus (S. aureus) based on copper-based metal-organic framework encapsulated with plenty of CuO nanocrystals (represented by ML-CuO@Cu-MOF) synthesized using the mixed ligands of diphenylethyne-3,3',5,5'-tetracarboxylic acid, 1,3,5-benzenetricarboxylic acid, and terephthalic acid. The ML-CuO@Cu-MOF nanospheres comprised multiple Cu valence states (Cu/Cu/Cu) and CuO nanocrystals, possessed defect-rich crystal structure, high anchoring ability toward aptamer and boosted electrical conductivity. The gained ML-CuO@Cu-MOF-based biosensor exhibited lower limit of detection toward S. aureus, as low as 2.0 and 1.6 CFU mL within the S. aureus concentration from 10 to 1 × 10 CFU mL obtained by electrochemical impedance spectroscopy and differential pulse voltammetry techniques, respectively. Additionally, the developed aptasensor also demonstrated high superior stability, excellent reproducibility, acceptable regeneration ability, and wide practicality. The present work can give rise to an alternative aptasensing strategy for analyzing foodborne bacteria or other food containments and ensuring food safety assessment.
我们提出了一种新颖的电化学适体传感策略，基于封装有大量 CuO 纳米晶体的铜基金属有机骨架（以 ML-CuO@Cu-MOF 表示），该策略通过使用二乙炔基-3,3',5,5'-四羧酸、1,3,5-苯三甲酸和对苯二甲酸的混合配体合成。ML-CuO@Cu-MOF 纳米球包含多种 Cu 价态（Cu/Cu/Cu）和 CuO 纳米晶体，具有富缺陷的晶体结构、对适体的高锚固能力和增强的导电性。所获得的基于 ML-CuO@Cu-MOF 的生物传感器对金黄色葡萄球菌表现出较低的检测下限，通过电化学阻抗谱和差分脉冲伏安法技术，在金黄色葡萄球菌浓度从 10 到 1×10 CFU mL 范围内，检测下限分别低至 2.0 和 1.6 CFU mL。此外，所开发的适体传感器还表现出高稳定性、优异的重现性、可接受的再生能力和广泛的实用性。本工作可以为分析食源性细菌或其他食品污染物以及确保食品安全评估提供一种替代的适体传感策略。
