Taylor H P, Armstrong S J, Dimmock N J
Virology. 1987 Aug;159(2):288-98. doi: 10.1016/0042-6822(87)90466-1.
In this quantitative study of the interaction of influenza virus with neutralizing antibody we have determined the maximum number of antibody molecules which can bind to the haemagglutinin (HA) of native influenza A/FPV/Rostock/34 (H7N1) particles in aqueous suspension and the minimum number which is required to cause neutralization. Using radiolabelled immunoglobulins approximately one IgG molecule, whether of monoclonal or polyclonal origin, binds per HA spike under conditions of antibody saturation. In the same manner, we have determined that when infectivity is neutralized by 63% (1/e) about 70 molecules of monoclonal IgGs HC2 and HC10 were bound per virus particle and this is supported by independent evidence from electron microscopy. However, the kinetics of neutralization were single-hit or at most, under critical conditions of low temperature (4 degrees) and minimal neutralizing concentrations of antibody, two-hit. This apparent conflict is reconciled by a hypothesis which proposes that neutralization occurs only when antibody binds to certain "neutralization relevant" HA spikes which are in the minority. It is suggested that these only differ from the majority of "neutralization irrelevant" HA spikes by their transmembrane interaction with the core of the virion.
在这项关于流感病毒与中和抗体相互作用的定量研究中，我们确定了在水悬浮液中能与天然甲型流感病毒A/FPV/Rostock/34（H7N1）颗粒的血凝素（HA）结合的抗体分子的最大数量，以及引起中和所需的最小数量。使用放射性标记的免疫球蛋白，在抗体饱和条件下，每个HA刺突大约结合一个IgG分子，无论其来源是单克隆还是多克隆。同样，我们确定，当感染性被中和63%（1/e）时，每个病毒颗粒大约结合70个单克隆IgG HC2和HC10分子，这得到了电子显微镜独立证据的支持。然而，中和动力学是单打击的，或者在低温（4摄氏度）和最低抗体中和浓度的临界条件下最多是双打击的。一个假设调和了这一明显的矛盾，该假设提出只有当抗体与少数“中和相关”的HA刺突结合时才会发生中和。有人认为，这些“中和相关”的HA刺突与大多数“中和无关”的HA刺突的区别仅在于它们与病毒粒子核心的跨膜相互作用。
