• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

A型肉毒杆菌毒素相关轴突运输在慢性压迫性损伤所致神经病理性疼痛中的作用

The role of botulinum toxin type A related axon transport in neuropathic pain induced by chronic constriction injury.

作者信息

Bu Huilian, Jiao Pengfei, Fan Xiaochong, Gao Yan, Zhang Lirong, Guo Haiming

机构信息

Center of Pain Management, Department of Anesthesiology, Pain and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Zhengzhou University Academy of Medical Sciences, Zhengzhou, China.

出版信息

Korean J Pain. 2022 Oct 1;35(4):391-402. doi: 10.3344/kjp.2022.35.4.391.

DOI:10.3344/kjp.2022.35.4.391
PMID:36175338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9530680/
Abstract

BACKGROUND

The mechanism of peripheral axon transport in neuropathic pain is still unclear. Chemokine ligand 13 (CXCL13) and its receptor (C-X-C chemokine receptor type 5, CXCR5) as well as GABA transporter 1 (GAT-1) play an important role in the development of pain. The aim of this study was to explore the axonal transport of CXCL13/CXCR5 and GAT-1 with the aid of the analgesic effect of botulinum toxin type A (BTX-A) in rats.

METHODS

Chronic constriction injury (CCI) rat models were established. BTX-A was administered to rats through subcutaneous injection in the hind paw. The pain behaviors in CCI rats were measured by paw withdrawal threshold and paw withdrawal latencies. The levels of CXCL13/CXCR5 and GAT-1 were measured by western blots.

RESULTS

The subcutaneous injection of BTX-A relieved the mechanical allodynia and heat hyperalgesia induced by CCI surgery and reversed the overexpression of CXCL13/CXCR5 and GAT-1 in the spinal cord, dorsal root ganglia (DRG), sciatic nerve, and plantar skin in CCI rats. After 10 mmol/L colchicine blocked the axon transport of sciatic nerve, the inhibitory effect of BTX-A disappeared, and the levels of CXCL13/CXCR5 and GAT-1 in the spinal cord and DRG were reduced in CCI rats.

CONCLUSIONS

BTX-A regulated the levels of CXCL13/CXCR5 and GAT-1 in the spine and DRG through axonal transport. Chemokines (such as CXCL13) may be transported from the injury site to the spine or DRG through axonal transport. Axon molecular transport may be a target to enhance pain management in neuropathic pain.

摘要

背景

神经性疼痛中周围轴突运输的机制仍不清楚。趋化因子配体13（CXCL13）及其受体（C-X-C趋化因子受体5型，CXCR5）以及γ-氨基丁酸转运体1（GAT-1）在疼痛的发生发展中起重要作用。本研究旨在借助A型肉毒毒素（BTX-A）对大鼠的镇痛作用，探讨CXCL13/CXCR5和GAT-1的轴突运输情况。

方法

建立慢性压迫损伤（CCI）大鼠模型。通过后爪皮下注射将BTX-A给予大鼠。采用爪退缩阈值和爪退缩潜伏期测量CCI大鼠的疼痛行为。通过蛋白质免疫印迹法检测CXCL13/CXCR5和GAT-1的水平。

结果

皮下注射BTX-A可缓解CCI手术诱导的机械性异常性疼痛和热痛觉过敏，并逆转CCI大鼠脊髓、背根神经节（DRG）、坐骨神经和足底皮肤中CXCL13/CXCR5和GAT-1的过表达。10 mmol/L秋水仙碱阻断坐骨神经的轴突运输后，BTX-A的抑制作用消失，CCI大鼠脊髓和DRG中CXCL13/CXCR5和GAT-1的水平降低。

结论

BTX-A通过轴突运输调节脊髓和DRG中CXCL13/CXCR5和GAT-1的水平。趋化因子（如CXCL13）可能通过轴突运输从损伤部位转运至脊髓或DRG。轴突分子运输可能是增强神经性疼痛疼痛管理的一个靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/310c973dd88a/kjp-35-4-391-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/f19bb3073477/kjp-35-4-391-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/12d1cd78000f/kjp-35-4-391-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/d7bc7904eec7/kjp-35-4-391-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/fe87aa23f201/kjp-35-4-391-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/9d630ef0871c/kjp-35-4-391-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/68f9b12f1e0f/kjp-35-4-391-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/310c973dd88a/kjp-35-4-391-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/f19bb3073477/kjp-35-4-391-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/12d1cd78000f/kjp-35-4-391-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/d7bc7904eec7/kjp-35-4-391-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/fe87aa23f201/kjp-35-4-391-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/9d630ef0871c/kjp-35-4-391-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/68f9b12f1e0f/kjp-35-4-391-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b2/9530680/310c973dd88a/kjp-35-4-391-f9.jpg

相似文献

1
The role of botulinum toxin type A related axon transport in neuropathic pain induced by chronic constriction injury.A型肉毒杆菌毒素相关轴突运输在慢性压迫性损伤所致神经病理性疼痛中的作用
Korean J Pain. 2022 Oct 1;35(4):391-402. doi: 10.3344/kjp.2022.35.4.391.
2
Mechanism of IRF5-regulated CXCL13/CXCR5 Signaling Axis in CCI-induced Neuropathic Pain in Rats.IRF5 调控的 CXCL13/CXCR5 信号轴在 CCI 诱导的大鼠神经病理性疼痛中的作用机制。
Curr Mol Med. 2024;24(7):940-949. doi: 10.2174/1566524023666230825120836.
3
MMP-9 regulates CX3CL1/CX3CR1 in the early phase of neuropathic pain in chronic sciatic nerve constriction injury (CCI) rats.基质金属蛋白酶-9在慢性坐骨神经缩窄损伤（CCI）大鼠神经性疼痛早期调节CX3CL1/CX3CR1。
Ann Palliat Med. 2020 Jul;9(4):2020-2027. doi: 10.21037/apm-20-1078. Epub 2020 Jul 13.
4
Pulsed Radiofrequency on Dorsal Root Ganglion Relieved Neuropathic Pain Associated with Downregulation of the Spinal Interferon Regulatory Factor 8, Microglia, p38MAPK Expression in a CCI Rat Model.脊神经根节脉冲射频缓解与脊髓干扰素调节因子 8、小胶质细胞、p38MAPK 表达下调相关的慢性压迫性损伤大鼠模型神经病理性疼痛。
Pain Physician. 2018 Jul;21(4):E307-E322.
5
Downregulations of TRPM8 expression and membrane trafficking in dorsal root ganglion mediate the attenuation of cold hyperalgesia in CCI rats induced by GFRα3 knockdown.下调背根神经节中 TRPM8 的表达和膜转运可介导 GFRα3 敲低诱导的 CCI 大鼠冷超敏反应的减弱。
Brain Res Bull. 2017 Oct;135:8-24. doi: 10.1016/j.brainresbull.2017.08.002. Epub 2017 Sep 1.
6
Upregulation of the GABA-transporter GAT-1 in the spinal cord contributes to pain behaviour in experimental neuropathy.脊髓中γ-氨基丁酸转运体GAT-1的上调促成了实验性神经病变中的疼痛行为。
Neurosci Lett. 2008 Oct 17;444(1):112-5. doi: 10.1016/j.neulet.2008.08.001. Epub 2008 Aug 5.
7
Involvement of hyperpolarization-activated, cyclic nucleotide-gated cation channels in dorsal root ganglion in neuropathic pain.超极化激活的环核苷酸门控阳离子通道在背根神经节参与神经性疼痛。
Sheng Li Xue Bao. 2008 Oct 25;60(5):579-80.
8
Chemokine CXCL13 mediates orofacial neuropathic pain via CXCR5/ERK pathway in the trigeminal ganglion of mice.趋化因子CXCL13通过CXCR5/ERK途径介导小鼠三叉神经节中的口面部神经性疼痛。
J Neuroinflammation. 2016 Jul 11;13(1):183. doi: 10.1186/s12974-016-0652-1.
9
Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve.坐骨神经单侧慢性压迫损伤后，双侧腰骶和颈背根神经节白细胞介素-6 蛋白和 mRNA 水平升高。
J Neuroinflammation. 2013 May 1;10:55. doi: 10.1186/1742-2094-10-55.
10
CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn.CXCL13 通过 CXCR5 介导的 NF-κB 激活和脊髓背角促炎细胞因子的产生，促进 CRPS-I 小鼠模型的慢性疼痛。
J Neuroinflammation. 2023 May 8;20(1):109. doi: 10.1186/s12974-023-02778-x.

引用本文的文献

1
Pioneering pain management with botulinum toxin type A: From anti-inflammation to regenerative therapies.A型肉毒杆菌毒素在疼痛管理方面的开创性应用：从抗炎到再生疗法。
Heliyon. 2025 Jan 28;11(4):e42350. doi: 10.1016/j.heliyon.2025.e42350. eCollection 2025 Feb 28.
2
Chemokine CXCL13-CXCR5 signaling in neuroinflammation and pathogenesis of chronic pain and neurological diseases.趋化因子 CXCL13-CXCR5 信号在神经炎症及慢性疼痛和神经疾病发病机制中的作用。
Cell Mol Biol Lett. 2024 Oct 29;29(1):134. doi: 10.1186/s11658-024-00653-y.
3
Neurobiological mechanisms of botulinum neurotoxin-induced analgesia for neuropathic pain.

本文引用的文献

1
The G Protein-Coupled Receptor Kinases (GRKs) in Chemokine Receptor-Mediated Immune Cell Migration: From Molecular Cues to Physiopathology.G 蛋白偶联受体激酶（GRKs）在趋化因子受体介导的免疫细胞迁移中的作用：从分子线索到病理生理学。
Cells. 2021 Jan 5;10(1):75. doi: 10.3390/cells10010075.
2
Molecular Basis for CCRL2 Regulation of Leukocyte Migration.CCRL2调控白细胞迁移的分子基础
Front Cell Dev Biol. 2020 Dec 10;8:615031. doi: 10.3389/fcell.2020.615031. eCollection 2020.
3
Retrospective Study on Ganglionic and Nerve Block Series as Therapeutic Option for Chronic Pain Patients with Refractory Neuropathic Pain.
肉毒杆菌神经毒素诱导的神经病理性疼痛镇痛的神经生物学机制。
Pharmacol Ther. 2024 Jul;259:108668. doi: 10.1016/j.pharmthera.2024.108668. Epub 2024 May 22.
4
Targeting Members of the Chemokine Family as a Novel Approach to Treating Neuropathic Pain.靶向趋化因子家族成员作为治疗神经病理性疼痛的新方法。
Molecules. 2023 Jul 30;28(15):5766. doi: 10.3390/molecules28155766.
回顾性研究：神经节和神经阻滞联合治疗对难治性神经病理性疼痛慢性疼痛患者的疗效。
Pain Res Manag. 2020 Jul 25;2020:6042941. doi: 10.1155/2020/6042941. eCollection 2020.
4
Mirogabalin in Japanese Patients with Renal Impairment and Pain Associated with Diabetic Peripheral Neuropathy or Post-Herpetic Neuralgia: A Phase III, Open-Label, 14-Week Study.米罗加巴林用于日本肾功能损害及伴有糖尿病性周围神经病变或带状疱疹后神经痛的疼痛患者：一项III期开放标签14周研究。
J Pain Res. 2020 Jul 17;13:1811-1821. doi: 10.2147/JPR.S255345. eCollection 2020.
5
Clinical Study of Spinal Cord Stimulation and Pulsed Radiofrequency for Management of Herpes Zoster-Related Pain Persisting Beyond Acute Phase in Elderly Patients.脊髓电刺激和脉冲射频治疗老年带状疱疹后急性期疼痛持续患者的临床研究。
Pain Physician. 2020 Jun;23(3):263-270.
6
Chemokines in chronic pain: cellular and molecular mechanisms and therapeutic potential.趋化因子在慢性疼痛中的作用：细胞和分子机制及治疗潜力。
Pharmacol Ther. 2020 Aug;212:107581. doi: 10.1016/j.pharmthera.2020.107581. Epub 2020 May 22.
7
A negative-feedback loop maintains optimal chemokine concentrations for directional cell migration.负反馈环维持趋化因子浓度以促进定向细胞迁移。
Nat Cell Biol. 2020 Mar;22(3):266-273. doi: 10.1038/s41556-020-0465-4. Epub 2020 Feb 10.
8
MiR-30b-5p attenuates oxaliplatin-induced peripheral neuropathic pain through the voltage-gated sodium channel Na1.6 in rats.miR-30b-5p 通过电压门控钠离子通道 Na1.6 减轻大鼠奥沙利铂诱导的周围神经性疼痛。
Neuropharmacology. 2019 Jul 15;153:111-120. doi: 10.1016/j.neuropharm.2019.04.024. Epub 2019 May 3.
9
HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats.组蛋白去乙酰化酶 2（HDAC2），而不是组蛋白去乙酰化酶 1（HDAC1），调节 Kv1.2 的表达，从而介导 CCI 大鼠的神经性疼痛。
Neuroscience. 2019 Jun 1;408:339-348. doi: 10.1016/j.neuroscience.2019.03.033. Epub 2019 Apr 23.
10
The Roles of Chemokine CXCL13 in the Development of Bone Cancer Pain and the Regulation of Morphine Analgesia in Rats.趋化因子 CXCL13 在大鼠癌痛发展中的作用及吗啡镇痛的调节。
Neuroscience. 2019 May 15;406:62-72. doi: 10.1016/j.neuroscience.2019.02.025. Epub 2019 Mar 1.