Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland.
The School of Biological Sciences, Monash University, Clayton, Melbourne, Victoria 3800, Australia.
Development. 2022 Oct 1;149(19). doi: 10.1242/dev.200580. Epub 2022 Oct 14.
Plant cells are surrounded by a cell wall, a rigid structure that is not only important for cell and organ shape, but is also crucial for intercellular communication and interactions with the environment. In the flowering plant Arabidopsis thaliana, the 17 members of the Catharanthus roseus RLK1-like (CrRLK1L) receptor kinase family are involved in a multitude of physiological and developmental processes, making it difficult to assess their primary or ancestral function. To reduce genetic complexity, we characterized the single CrRLK1L gene of Marchantia polymorpha, MpFERONIA (MpFER). Plants with reduced MpFER levels show defects in vegetative development, i.e. rhizoid formation and cell expansion, and have reduced male fertility. In contrast, cell integrity and morphogenesis of the gametophyte are severely affected in Mpfer null mutants and MpFER overexpression lines. Thus, we conclude that the CrRLK1L gene family originated from a single gene with an ancestral function in cell expansion and the maintenance of cellular integrity. During land plant evolution, this ancestral gene diversified to fulfill a multitude of specialized physiological and developmental roles in the formation of both gametophytic and sporophytic structures essential to the life cycle of flowering plants.
植物细胞被细胞壁包围，细胞壁是一种刚性结构，不仅对细胞和器官的形状很重要，而且对细胞间的通讯和与环境的相互作用也至关重要。在开花植物拟南芥中，17 个长春花受体样激酶 1 样（CrRLK1L）受体激酶家族成员参与了多种生理和发育过程，因此很难评估它们的主要或原始功能。为了降低遗传复杂性，我们对厚叶卷柏的单个 CrRLK1L 基因 MpFERONIA（MpFER）进行了表征。MpFER 水平降低的植物在营养生长发育中表现出缺陷，即假根形成和细胞扩张，并且雄性育性降低。相比之下，Mpfer 缺失突变体和 MpFER 过表达系的配子体的细胞完整性和形态发生受到严重影响。因此，我们得出结论，CrRLK1L 基因家族起源于一个具有细胞扩张和维持细胞完整性的原始功能的单一基因。在陆地植物进化过程中，这个祖先基因多样化，以在形成配子体和孢子体结构中发挥多种专门的生理和发育作用，这些结构对有花植物的生命周期至关重要。
