Kang Chang Muk, Bang Joon Seok, Park Seung Yeon, Jung Tae Woo, Kim Hyoung-Chun, Chung Yoon Hee, Jeong Ji Hoon
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Korea.
College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
J Med Food. 2022 Oct;25(10):943-951. doi: 10.1089/jmf.2021.K.0197. Epub 2022 Sep 29.
(HJ) is an herbal medicine, which has been reported as being antioxidative and anti-inflammatory. The present study aimed to investigate the effect of oral administration of HJ water extract (HJW) on cognitive function through the cholinergic system in Alzheimer's disease (AD) mouse models. Institute of Cancer Research mice injected with beta-amyloid (A) (1-42) (i.c.v.) and APP/PS1 transgenic (TG) mice were orally administered with HJW at 500 mg/kg/day for 3 weeks. A-injected mice and APP/PS1 TG mice showed cognitive dysfunction, which was evaluated by various behavioral tests. HJW treatment significantly attenuated memory impairments in A-injected mice and APP/PS1 TG mice. A injection decreased acetylcholine (ACh) concentrations and choline acetyltransferase (ChAT) activity, and increased acetylcholinesterase (AChE) activity. These cholinergic impairments were also found in APP/PS1 TG mice. HJW significantly attenuated cholinergic alterations in A-injected mice and TG mice. In addition, HJW significantly decreased A plaque deposition in the cerebral cortex and hippocampus of TG mice. Therefore, the present study demonstrated that HJW protected against AD-related memory impairments via enhancing the cholinergic system and inhibiting A plaque deposition.
（HJ）是一种草药，据报道具有抗氧化和抗炎作用。本研究旨在通过阿尔茨海默病（AD）小鼠模型中的胆碱能系统，研究口服HJ水提取物（HJW）对认知功能的影响。给注射β-淀粉样蛋白（A）（1-42）（脑室内注射）的癌症研究所小鼠和APP/PS1转基因（TG）小鼠，以500mg/kg/天的剂量口服HJW，持续3周。注射A的小鼠和APP/PS1 TG小鼠表现出认知功能障碍，通过各种行为测试进行评估。HJW治疗显著减轻了注射A的小鼠和APP/PS1 TG小鼠的记忆损伤。注射A降低了乙酰胆碱（ACh）浓度和胆碱乙酰转移酶（ChAT）活性，并增加了乙酰胆碱酯酶（AChE）活性。在APP/PS1 TG小鼠中也发现了这些胆碱能损伤。HJW显著减轻了注射A的小鼠和TG小鼠的胆碱能改变。此外，HJW显著减少了TG小鼠大脑皮层和海马体中的A斑块沉积。因此，本研究表明，HJW通过增强胆碱能系统和抑制A斑块沉积，预防与AD相关的记忆损伤。
