Blasco Lucia, Kahala Minna, Ervasti Satu, Tampio Elina
Natural Resources Institute Finland (Luke), Production Systems, Myllytie 1, FI-31600 Jokioinen, Finland.
Natural Resources Institute Finland (Luke), Production Systems, Myllytie 1, FI-31600 Jokioinen, Finland.
Bioresour Technol. 2022 Nov;364:128039. doi: 10.1016/j.biortech.2022.128039. Epub 2022 Sep 29.
To enable the utilization of seasonal biomasses in e.g., farm-scale biogas plants, the process should be flexible and ensure stable gas production. However, information about microbial community dynamics in long-term co-digestion with versatile co-feedstocks is lacking. This study investigated the effects of co-feedstock changes on the performance and evolution of microbial consortia during 428-day anaerobic digestion of cow slurry. Co-feedstocks consisted of hydrocarbon-, protein- and lipid-rich materials. A high throughput 16S ribosomal RNA gene sequencing was used to analyze the taxonomic profile of microbial communities. Due to the low loading rate, the changes were subtle in bacteria, but a shift on archaeal genera in response to different and changing feedstock compositions was observed. Despite drastic changes in co-feedstock composition, stable and flexible anaerobic digestion with relatively constant core microbiome can be achieved with cautious operation of the process.
为了能够在农场规模的沼气厂中利用季节性生物质，该工艺应具有灵活性并确保稳定的气体生产。然而，缺乏关于与多种共原料长期共消化过程中微生物群落动态的信息。本研究调查了在牛粪浆428天厌氧消化过程中，共原料变化对微生物群落性能和演变的影响。共原料包括富含碳氢化合物、蛋白质和脂质的材料。采用高通量16S核糖体RNA基因测序分析微生物群落的分类概况。由于负荷率较低，细菌的变化较为细微，但观察到古菌属因不同且不断变化的原料组成而发生了转变。尽管共原料组成发生了剧烈变化，但通过谨慎操作该工艺，仍可实现具有相对恒定核心微生物组的稳定且灵活的厌氧消化。
