This study investigated the effects of two probiotics namely Clostridium butyricum and Bacillus subtilis, and one prebiotic known as algae-sourced β-1,3 glucan, on the overall performances of grass turtles (Chinemys reevesii) juveniles. Growth performance, immune responses, enzymatic antioxidant activities, intestinal histomorphology, and disease resistance against the challenge with Aeromonas veronii were assessed. Two hundred and sixteen (216) juvenile turtles with an average initial weight of 106.35 ± 0.03 g were divided into four groups, each containing three replicates with 18 turtles per each replicate, which were fed a basic diet (control group, GD) and a basal diet supplemented with C. butyricum 1.0 × 10 CFU per kg (GA group), or with B. subtilis 1.0 × 10 CFU per kg (GB group) and with algal-sourced β-1,3-glucan 50 mg per kg (GC group), respectively. After the turtles had been fed for 60 d, 90 d, and 120 d of the experimental period, the growth performance and survival rate (SR), intestinal digestive enzyme, hepatic and intestinal antioxidant capacity, serum biochemical indexes, and immune performance were measured. The results showed that the weight gain rate and SR were significantly enhanced (P < 0.05) after fed probiotics and algae-sourced β-1,3-glucan in all test times;The pepsin, amylase, acid phosphatase, total antioxidant capacity, triglyceride, alkaline phosphatase, urea nitrogen, cholesterol, total protein, IgA, IgG, IgM at 120 d were significantly enhanced (P<0.05) after fed C. butyricum. The intestinal villi heights, widths, and the thickness of the muscle layer were significantly higher in groups GA, GB, and GC than those reared within the GD control group (P < 0.05). After injecting the challenge by A. veronii the survival rate of grass turtles in the GA group (75%) was significantly higher than the other three groups (P<0.05), while there was no significant difference between the GB and GC groups compared with the control GD group, respectively (P>0.05). Overall, these results indicated that dietary supplementation with probiotics or algae-sourced β-1,3 glucan, exhibited positive effects on C. reevesii. In particular, C. butyricum, showed the greatest improvements relating to growth, immune response, antioxidant activity, intestinal health, and disease resistance.