Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Freie Universität Berlin, Institute for Biology, Berlin, Germany.
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, Germany.
STAR Protoc. 2022 Dec 16;3(4):101731. doi: 10.1016/j.xpro.2022.101731. Epub 2022 Sep 30.
The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated or inhibited by the stressor using a relatively rapid immunofluorescence staining approach. This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation. For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022).
幼期斑马鱼已成为研究控制神经内分泌和应激行为反应的神经元回路的非常有用的模式生物。本方案描述了如何使斑马鱼幼体暴露于高渗应激下，并使用相对快速的免疫荧光染色方法来测试候选神经元群体是否被应激原激活或抑制。这种方法利用了神经元激活时细胞外信号调节激酶（ERK）的磷酸化。有关该方案的使用和执行的完整详细信息，请参考 Corradi 等人（2022 年）。
