Department of Pathology, Dalhousie University, Sir Charles Tupper Medical Building, 5850 College St., Halifax, NS B3H 4H7, Canada.
Department of Microbiology and Immunology, Dalhousie University, Room 7C, Sir Charles Tupper Medical Building, 5850 College St., Halifax, NS B3H 4H7, Canada; Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
STAR Protoc. 2022 Dec 16;3(4):101738. doi: 10.1016/j.xpro.2022.101738. Epub 2022 Sep 30.
Mitochondrial damage-associated molecular patterns (mitoDAMPs) are released from cells dying uncontrolled, non-apoptotic deaths, usually secondary to disease or trauma. Here, we describe preparation of mitoDAMPs from mouse liver, but this protocol can be adapted for preparation of mitoDAMPs from other species and tissues. Tissues are dissociated and then processed to isolate mitochondria. Mitochondria are then sonicated and mitoDAMPs are collected by ultracentrifugation. This procedure produces μg quantities of mitoDAMPs and facilitates research to understand their impacts in health and disease. For complete details on the use and execution of this protocol, please refer to Westhaver et al. (2022).
线粒体损伤相关分子模式（mitoDAMPs）是从失控的、非凋亡性死亡的细胞中释放出来的，通常是疾病或创伤的继发反应。在这里，我们描述了从小鼠肝脏中制备 mitoDAMPs 的方法，但该方案可以适用于从其他物种和组织中制备 mitoDAMPs。组织被分离，然后进行处理以分离线粒体。然后对线粒体进行超声处理，并通过超速离心收集 mitoDAMPs。该步骤可产生 μg 级别的 mitoDAMPs，有助于研究它们在健康和疾病中的影响。有关该方案的使用和执行的完整详细信息，请参阅 Westhaver 等人（2022 年）。
